Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila

Shunya Hozumi, Reo Maeda, Maiko Taniguchi-Kanai, Takashi Okumura, Kiichiro Taniguchi, Yasuhiro Kawakatsu, Naotaka Nakazawa, Ryo Hatori, Kenji Matsuno

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In Drosophila, Myosin31DF (Myo31DF), encoding a Myosin ID protein, has crucial roles in left-right (LR) asymmetric development. Loss of Myo31DF function leads to laterality inversion for many organs, including the embryonic gut. Here, we found that Myo31DF was required before LR asymmetric morphogenesis in the hindgut, suggesting it functions in LR patterning instead of directly in hindgut morphological changes. Myosin61F (Myo61F) encodes another Myosin I, and Myo31DF or Myo61F overexpression reverses the laterality of different organs. Myo31DF and Myo61F have domains conserved in Myosin proteins, particularly in the proteins' head regions. We studied the roles of these domains in LR patterning using overexpression analysis. The Actin-binding and ATP-binding domains were essential for both proteins, but the IQ domains, binding sites for Myosin light chains, were required only by Myo31DF. Our results also suggest that the organ specificities of the Myo31DF and Myo61F activities depended on their head regions.

Original languageEnglish
Pages (from-to)3528-3537
Number of pages10
JournalDevelopmental Dynamics
Volume237
Issue number12
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

Fingerprint

Myosin Type I
Organ Specificity
Drosophila
Head
Myosins
Myosin Light Chains
Proteins
Morphogenesis
Binding Sites

Keywords

  • Drosophila melanogaster
  • Left-right asymmetry
  • Myosin I
  • Myosin31DF
  • Myosin61F

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Hozumi, S., Maeda, R., Taniguchi-Kanai, M., Okumura, T., Taniguchi, K., Kawakatsu, Y., ... Matsuno, K. (2008). Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila. Developmental Dynamics, 237(12), 3528-3537. https://doi.org/10.1002/dvdy.21583

Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila. / Hozumi, Shunya; Maeda, Reo; Taniguchi-Kanai, Maiko; Okumura, Takashi; Taniguchi, Kiichiro; Kawakatsu, Yasuhiro; Nakazawa, Naotaka; Hatori, Ryo; Matsuno, Kenji.

In: Developmental Dynamics, Vol. 237, No. 12, 12.2008, p. 3528-3537.

Research output: Contribution to journalArticle

Hozumi, S, Maeda, R, Taniguchi-Kanai, M, Okumura, T, Taniguchi, K, Kawakatsu, Y, Nakazawa, N, Hatori, R & Matsuno, K 2008, 'Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila', Developmental Dynamics, vol. 237, no. 12, pp. 3528-3537. https://doi.org/10.1002/dvdy.21583
Hozumi, Shunya ; Maeda, Reo ; Taniguchi-Kanai, Maiko ; Okumura, Takashi ; Taniguchi, Kiichiro ; Kawakatsu, Yasuhiro ; Nakazawa, Naotaka ; Hatori, Ryo ; Matsuno, Kenji. / Head region of unconventional myosin I family members is responsible for the organ-specificity of their roles in left-right polarity in Drosophila. In: Developmental Dynamics. 2008 ; Vol. 237, No. 12. pp. 3528-3537.
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