Heat-resistant oxygen-carrying hemoproteins consist of recombinant xylanases and synthetic iron(II) porphyrin

Teruyuki Komatsu, Seiji Ishihara, Eishun Tsuchida, Hiroyuki Nishide, Chihiro Morokuma, Satoshi Nakamura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Synthetic iron(II) porphyrin (FeP) is equivalently incorporated into recombinant Thermotoga maritima xylanase B (TMX; family F/10 of glycoside hydrolase), producing a heat-resistant artificial hemoprotein (TMX-FeP) that can bind and release oxygen (O2) in aqueous medium (pH 7.3, 25 °C) in the same manner as hemoglobin and myoglobin. The oxygenated species was sufficiently stable; the half-lifetime against the ferric state (τ1/2) was 5 h. This O2-carrying hemoprotein showed a high degree of thermal stability over a wide range of temperatures up to 90 °C (τ1/2 = 5 min at 90 °C and 9 min at 75 °C). Dictyoglomus thermophilum xylanase B (DTX; family G/11) also incorporates FeP, and DTX-FeP showed identical O2-binding parameters and thermostability. TMX-FeP is capable of catalyzing the β-1,4-D-xylan hydrolysis reaction. Its larger Km value compared to that of TMX itself suggested competitive FeP binding to the active site of the host enzyme.

Original languageEnglish
Pages (from-to)1489-1494
Number of pages6
JournalBiomacromolecules
Volume6
Issue number3
DOIs
Publication statusPublished - 2005 May

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Endo-1,4-beta Xylanases
Hemoglobin
Porphyrins
Hydrolysis
Thermodynamic stability
Iron
Enzymes
Hot Temperature
Thermotoga maritima
Oxygen
Xylans
Competitive Binding
Myoglobin
Glycoside Hydrolases
Catalytic Domain
Hemoglobins
Temperature
(1-4)-xylan

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Heat-resistant oxygen-carrying hemoproteins consist of recombinant xylanases and synthetic iron(II) porphyrin. / Komatsu, Teruyuki; Ishihara, Seiji; Tsuchida, Eishun; Nishide, Hiroyuki; Morokuma, Chihiro; Nakamura, Satoshi.

In: Biomacromolecules, Vol. 6, No. 3, 05.2005, p. 1489-1494.

Research output: Contribution to journalArticle

Komatsu, Teruyuki ; Ishihara, Seiji ; Tsuchida, Eishun ; Nishide, Hiroyuki ; Morokuma, Chihiro ; Nakamura, Satoshi. / Heat-resistant oxygen-carrying hemoproteins consist of recombinant xylanases and synthetic iron(II) porphyrin. In: Biomacromolecules. 2005 ; Vol. 6, No. 3. pp. 1489-1494.
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