Heat-stability of iron-sulfur centers and P-700 in photosystem I reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus

Kintake Sonoike; Hideki Hatanaka; Sakae Katoh; Shigeru Itoh

Heat-stability of iron-sulfur centers and P-700 in photosystem I reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus

Kintake Sonoike^*, Hideki Hatanaka, Sakae Katoh, Shigeru Itoh

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Stabilities of iron-sulfur centers and reaction center chlorophyll P-700 in Photosystem I reaction center complex (CP1-a), isolated by sodium dodecyl sulfate treatment from the thermophilic cyanobacterium Synechococcus elongatus, were studied by EPR and optical spectroscopy. P-700 was destroyed by treatment at temperatures above 80°C for 5 minutes with a half inactivation temperature of 93°C. The three iron-sulfur centers F_A, F_B and F_X showed similar thermal stabilities and were half inactivated at about 70°C. Thus, the isolated Photosystem I reaction center complexes of S. elongatus are still highly resistant to heat.

Original language	English
Pages (from-to)	865-870
Number of pages	6
Journal	Plant and Cell Physiology
Volume	31
Issue number	6
Publication status	Published - 1990
Externally published	Yes

Keywords

EPR spectroscopy
Heat-stability
Iron-sulfur centers
Photosystem I
Reaction center complex
Synechococcus elongatus

ASJC Scopus subject areas

Physiology
Plant Science
Cell Biology

Cite this

@article{2c39cdce60c943c29b6de33d19ee824b,

title = "Heat-stability of iron-sulfur centers and P-700 in photosystem I reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus",

abstract = "Stabilities of iron-sulfur centers and reaction center chlorophyll P-700 in Photosystem I reaction center complex (CP1-a), isolated by sodium dodecyl sulfate treatment from the thermophilic cyanobacterium Synechococcus elongatus, were studied by EPR and optical spectroscopy. P-700 was destroyed by treatment at temperatures above 80°C for 5 minutes with a half inactivation temperature of 93°C. The three iron-sulfur centers FA, FB and FX showed similar thermal stabilities and were half inactivated at about 70°C. Thus, the isolated Photosystem I reaction center complexes of S. elongatus are still highly resistant to heat.",

keywords = "EPR spectroscopy, Heat-stability, Iron-sulfur centers, Photosystem I, Reaction center complex, Synechococcus elongatus",

author = "Kintake Sonoike and Hideki Hatanaka and Sakae Katoh and Shigeru Itoh",

note = "Funding Information: The authors thank Miss M. Iwaki of N.I.B.B. and Dr. I. Ikegami of Teikyo University for their kind suggestions and technical help for the EPR measurements and Dr. Y. Fujita of N.I.B.B. for his support for this study. The work was supported in part by grants for Scientific Research from the Ministry of Education, Science and Culture, Japan to S. I. and S. K.",

year = "1990",

language = "English",

volume = "31",

pages = "865--870",

journal = "Plant and Cell Physiology",

issn = "0032-0781",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - Heat-stability of iron-sulfur centers and P-700 in photosystem I reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus

AU - Sonoike, Kintake

AU - Hatanaka, Hideki

AU - Katoh, Sakae

AU - Itoh, Shigeru

N1 - Funding Information: The authors thank Miss M. Iwaki of N.I.B.B. and Dr. I. Ikegami of Teikyo University for their kind suggestions and technical help for the EPR measurements and Dr. Y. Fujita of N.I.B.B. for his support for this study. The work was supported in part by grants for Scientific Research from the Ministry of Education, Science and Culture, Japan to S. I. and S. K.

PY - 1990

Y1 - 1990

N2 - Stabilities of iron-sulfur centers and reaction center chlorophyll P-700 in Photosystem I reaction center complex (CP1-a), isolated by sodium dodecyl sulfate treatment from the thermophilic cyanobacterium Synechococcus elongatus, were studied by EPR and optical spectroscopy. P-700 was destroyed by treatment at temperatures above 80°C for 5 minutes with a half inactivation temperature of 93°C. The three iron-sulfur centers FA, FB and FX showed similar thermal stabilities and were half inactivated at about 70°C. Thus, the isolated Photosystem I reaction center complexes of S. elongatus are still highly resistant to heat.

AB - Stabilities of iron-sulfur centers and reaction center chlorophyll P-700 in Photosystem I reaction center complex (CP1-a), isolated by sodium dodecyl sulfate treatment from the thermophilic cyanobacterium Synechococcus elongatus, were studied by EPR and optical spectroscopy. P-700 was destroyed by treatment at temperatures above 80°C for 5 minutes with a half inactivation temperature of 93°C. The three iron-sulfur centers FA, FB and FX showed similar thermal stabilities and were half inactivated at about 70°C. Thus, the isolated Photosystem I reaction center complexes of S. elongatus are still highly resistant to heat.

KW - EPR spectroscopy

KW - Heat-stability

KW - Iron-sulfur centers

KW - Photosystem I

KW - Reaction center complex

KW - Synechococcus elongatus

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M3 - Article

AN - SCOPUS:0001346919

SN - 0032-0781

VL - 31

SP - 865

EP - 870

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

IS - 6

ER -

Heat-stability of iron-sulfur centers and P-700 in photosystem I reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus

Abstract

Keywords

ASJC Scopus subject areas

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