Heat-stability of iron-sulfur centers and P-700 in photosystem I reaction center complexes isolated from the thermophilic cyanobacterium synechococcus elongatus

Kintake Sonoike, Hideki Hatanaka, Sakae Katoh, Shigeru Itoh

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

Stabilities of iron-sulfur centers and reaction center chlorophyll P-700 in Photosystem I reaction center complex (CP1-a), isolated by sodium dodecyl sulfate treatment from the thermophilic cyanobacterium Synechococcus elongatus, were studied by EPR and optical spectroscopy. P-700 was destroyed by treatment at temperatures above 80°C for 5 minutes with a half inactivation temperature of 93°C. The three iron-sulfur centers FA, FB and FX showed similar thermal stabilities and were half inactivated at about 70°C. Thus, the isolated Photosystem I reaction center complexes of S. elongatus are still highly resistant to heat.

Original languageEnglish
Pages (from-to)865-870
Number of pages6
JournalPlant and Cell Physiology
Volume31
Issue number6
Publication statusPublished - 1990 Dec 1
Externally publishedYes

Keywords

  • EPR spectroscopy
  • Heat-stability
  • Iron-sulfur centers
  • Photosystem I
  • Reaction center complex
  • Synechococcus elongatus

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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