Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats

Hiromi Sakai, Yohei Masada, Hirohisa Horinouchi, Manabu Yamamoto, Eiji Ikeda, Shinji Takeoka, Koichi Kobayashi, Eishun Tsuchida

    Research output: Contribution to journalArticle

    95 Citations (Scopus)

    Abstract

    Objective: Hemoglobin-vesicle (HbV) has been developed to provide oxygen-carrying ability to plasma expanders. Its ability to restore the systemic condition after hemorrhagic shock was evaluated in anesthetized Wistar rats for 6 hrs after resuscitation. The HbV was suspended in 5 g/dL recombinant human serum albumin (HbV/rHSA) at an Hb concentration of 8.6 g/dL. Design: Prospective, randomized, controlled trial. Setting: Department of Surgery, School of Medicine, Keio University. Subjects: Forty male Wistar rats. Interventions: The rats were anesthetized with 1.5% sevoflurane inhalation throughout the experiment Polyethylene catheters were introduced through the right jugular vein into the right atrium for infusion and into the right common carotid artery for blood withdrawal and mean arterial pressure monitoring. Measurements and Main Results: Shock was induced by 50% blood withdrawal. The rats showed hypotension (mean arterial pressure = 32 ± 10 mm Hg) and significant metabolic acidosis and hyperventilation. After 15 mins, they received HbV/rHSA, shed autologous blood (SAB), washed homologous red blood cells (wRBC) suspended in rHSA (wRBC/rHSA, [Hb] = 8.6 g/dL), or rHSA alone. The HbV/rHSA group restored mean arterial pressure to 93 ± 8 mm Hg at 1 hr, similar to the SAB group (92 ± 9 mm Hg), which was significantly higher compared with the rHSA (74 ± 9 mm Hg) and wRBC/rHSA (79 ± 8 mm Hg) groups. There was no remarkable difference in the blood gas variables between the resuscitated groups; however, two of eight rats in the rHSA group died before 6 hrs. After 6 hrs, the rHSA group showed significant ischemic changes in the right cerebral hemisphere relating to the ligation of the right carotid artery followed by cannulation, whereas the HbV/rHSA, SAB, and wRBC/rHSA groups showed less changes. Conclusions: HbV suspended in recombinant human serum albumin provides restoration from hemorrhagic shock that is comparable with that using shed autologous blood.

    Original languageEnglish
    Pages (from-to)539-545
    Number of pages7
    JournalCritical Care Medicine
    Volume32
    Issue number2
    DOIs
    Publication statusPublished - 2004 Feb

    Fingerprint

    Hemorrhagic Shock
    Resuscitation
    Serum Albumin
    Hemoglobins
    Erythrocytes
    Arterial Pressure
    Wistar Rats
    Hyperventilation
    Common Carotid Artery
    Jugular Veins
    Cerebrum
    Polyethylene
    Blood Group Antigens
    Acidosis
    Heart Atria
    Carotid Arteries
    Catheterization
    Hypotension
    Inhalation
    Ligation

    Keywords

    • Artificial red cells
    • Blood substitutes
    • Liposome
    • Resuscitation
    • Transfusion

    ASJC Scopus subject areas

    • Critical Care and Intensive Care Medicine

    Cite this

    Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats. / Sakai, Hiromi; Masada, Yohei; Horinouchi, Hirohisa; Yamamoto, Manabu; Ikeda, Eiji; Takeoka, Shinji; Kobayashi, Koichi; Tsuchida, Eishun.

    In: Critical Care Medicine, Vol. 32, No. 2, 02.2004, p. 539-545.

    Research output: Contribution to journalArticle

    Sakai, Hiromi ; Masada, Yohei ; Horinouchi, Hirohisa ; Yamamoto, Manabu ; Ikeda, Eiji ; Takeoka, Shinji ; Kobayashi, Koichi ; Tsuchida, Eishun. / Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats. In: Critical Care Medicine. 2004 ; Vol. 32, No. 2. pp. 539-545.
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    abstract = "Objective: Hemoglobin-vesicle (HbV) has been developed to provide oxygen-carrying ability to plasma expanders. Its ability to restore the systemic condition after hemorrhagic shock was evaluated in anesthetized Wistar rats for 6 hrs after resuscitation. The HbV was suspended in 5 g/dL recombinant human serum albumin (HbV/rHSA) at an Hb concentration of 8.6 g/dL. Design: Prospective, randomized, controlled trial. Setting: Department of Surgery, School of Medicine, Keio University. Subjects: Forty male Wistar rats. Interventions: The rats were anesthetized with 1.5{\%} sevoflurane inhalation throughout the experiment Polyethylene catheters were introduced through the right jugular vein into the right atrium for infusion and into the right common carotid artery for blood withdrawal and mean arterial pressure monitoring. Measurements and Main Results: Shock was induced by 50{\%} blood withdrawal. The rats showed hypotension (mean arterial pressure = 32 ± 10 mm Hg) and significant metabolic acidosis and hyperventilation. After 15 mins, they received HbV/rHSA, shed autologous blood (SAB), washed homologous red blood cells (wRBC) suspended in rHSA (wRBC/rHSA, [Hb] = 8.6 g/dL), or rHSA alone. The HbV/rHSA group restored mean arterial pressure to 93 ± 8 mm Hg at 1 hr, similar to the SAB group (92 ± 9 mm Hg), which was significantly higher compared with the rHSA (74 ± 9 mm Hg) and wRBC/rHSA (79 ± 8 mm Hg) groups. There was no remarkable difference in the blood gas variables between the resuscitated groups; however, two of eight rats in the rHSA group died before 6 hrs. After 6 hrs, the rHSA group showed significant ischemic changes in the right cerebral hemisphere relating to the ligation of the right carotid artery followed by cannulation, whereas the HbV/rHSA, SAB, and wRBC/rHSA groups showed less changes. Conclusions: HbV suspended in recombinant human serum albumin provides restoration from hemorrhagic shock that is comparable with that using shed autologous blood.",
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    T1 - Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats

    AU - Sakai, Hiromi

    AU - Masada, Yohei

    AU - Horinouchi, Hirohisa

    AU - Yamamoto, Manabu

    AU - Ikeda, Eiji

    AU - Takeoka, Shinji

    AU - Kobayashi, Koichi

    AU - Tsuchida, Eishun

    PY - 2004/2

    Y1 - 2004/2

    N2 - Objective: Hemoglobin-vesicle (HbV) has been developed to provide oxygen-carrying ability to plasma expanders. Its ability to restore the systemic condition after hemorrhagic shock was evaluated in anesthetized Wistar rats for 6 hrs after resuscitation. The HbV was suspended in 5 g/dL recombinant human serum albumin (HbV/rHSA) at an Hb concentration of 8.6 g/dL. Design: Prospective, randomized, controlled trial. Setting: Department of Surgery, School of Medicine, Keio University. Subjects: Forty male Wistar rats. Interventions: The rats were anesthetized with 1.5% sevoflurane inhalation throughout the experiment Polyethylene catheters were introduced through the right jugular vein into the right atrium for infusion and into the right common carotid artery for blood withdrawal and mean arterial pressure monitoring. Measurements and Main Results: Shock was induced by 50% blood withdrawal. The rats showed hypotension (mean arterial pressure = 32 ± 10 mm Hg) and significant metabolic acidosis and hyperventilation. After 15 mins, they received HbV/rHSA, shed autologous blood (SAB), washed homologous red blood cells (wRBC) suspended in rHSA (wRBC/rHSA, [Hb] = 8.6 g/dL), or rHSA alone. The HbV/rHSA group restored mean arterial pressure to 93 ± 8 mm Hg at 1 hr, similar to the SAB group (92 ± 9 mm Hg), which was significantly higher compared with the rHSA (74 ± 9 mm Hg) and wRBC/rHSA (79 ± 8 mm Hg) groups. There was no remarkable difference in the blood gas variables between the resuscitated groups; however, two of eight rats in the rHSA group died before 6 hrs. After 6 hrs, the rHSA group showed significant ischemic changes in the right cerebral hemisphere relating to the ligation of the right carotid artery followed by cannulation, whereas the HbV/rHSA, SAB, and wRBC/rHSA groups showed less changes. Conclusions: HbV suspended in recombinant human serum albumin provides restoration from hemorrhagic shock that is comparable with that using shed autologous blood.

    AB - Objective: Hemoglobin-vesicle (HbV) has been developed to provide oxygen-carrying ability to plasma expanders. Its ability to restore the systemic condition after hemorrhagic shock was evaluated in anesthetized Wistar rats for 6 hrs after resuscitation. The HbV was suspended in 5 g/dL recombinant human serum albumin (HbV/rHSA) at an Hb concentration of 8.6 g/dL. Design: Prospective, randomized, controlled trial. Setting: Department of Surgery, School of Medicine, Keio University. Subjects: Forty male Wistar rats. Interventions: The rats were anesthetized with 1.5% sevoflurane inhalation throughout the experiment Polyethylene catheters were introduced through the right jugular vein into the right atrium for infusion and into the right common carotid artery for blood withdrawal and mean arterial pressure monitoring. Measurements and Main Results: Shock was induced by 50% blood withdrawal. The rats showed hypotension (mean arterial pressure = 32 ± 10 mm Hg) and significant metabolic acidosis and hyperventilation. After 15 mins, they received HbV/rHSA, shed autologous blood (SAB), washed homologous red blood cells (wRBC) suspended in rHSA (wRBC/rHSA, [Hb] = 8.6 g/dL), or rHSA alone. The HbV/rHSA group restored mean arterial pressure to 93 ± 8 mm Hg at 1 hr, similar to the SAB group (92 ± 9 mm Hg), which was significantly higher compared with the rHSA (74 ± 9 mm Hg) and wRBC/rHSA (79 ± 8 mm Hg) groups. There was no remarkable difference in the blood gas variables between the resuscitated groups; however, two of eight rats in the rHSA group died before 6 hrs. After 6 hrs, the rHSA group showed significant ischemic changes in the right cerebral hemisphere relating to the ligation of the right carotid artery followed by cannulation, whereas the HbV/rHSA, SAB, and wRBC/rHSA groups showed less changes. Conclusions: HbV suspended in recombinant human serum albumin provides restoration from hemorrhagic shock that is comparable with that using shed autologous blood.

    KW - Artificial red cells

    KW - Blood substitutes

    KW - Liposome

    KW - Resuscitation

    KW - Transfusion

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