Hemolysis of human erythrocytes by a transient electric field

K. Kinosita, T. Y. Tsong

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Exposure of human erythrocytes, under isotonic conditions, to a high voltage pulse of a few kV/cm leads to total hemolysis of the red cells. Experiments described herein demonstrate that the hemolysis is due to the effect of the electric field. Neither the effect of current nor the extent of the rapid Joule-heating to the suspending medium shows a direct correlation with the observed hemolysis. Voltage pulsation of the erythrocyte suspension can induce a transmembrane potential across the cell membrane and, at a critical point, it either opens up or creates pores in the red cells. In isotonic saline the pores are small. They allow passage of potassium and sodium ions but not sucrose and hemoglobin molecules. The pores are larger in low ionic conditions, and permit permeation of sucrose molecules, but under no circumstances can hemoglobin leak out as the direct result of the voltage pulse. Kinetic measurements indicate that the hemolysis of the red cells follows a stepwise mechanism: leakage of ions leads to an osmotic imbalance which in turn causes a colloidal hemolysis of the red cells. Other effects of the voltage pulsation are also discussed.

Original languageEnglish
Pages (from-to)1923-1927
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number5
Publication statusPublished - 1977
Externally publishedYes

Fingerprint

Hemolysis
Erythrocytes
Sucrose
Hemoglobins
Ions
Membrane Potentials
Heating
Suspensions
Potassium
Sodium
Cell Membrane

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Hemolysis of human erythrocytes by a transient electric field. / Kinosita, K.; Tsong, T. Y.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 74, No. 5, 1977, p. 1923-1927.

Research output: Contribution to journalArticle

@article{4934a6d802a843869b43fbd278600e69,
title = "Hemolysis of human erythrocytes by a transient electric field",
abstract = "Exposure of human erythrocytes, under isotonic conditions, to a high voltage pulse of a few kV/cm leads to total hemolysis of the red cells. Experiments described herein demonstrate that the hemolysis is due to the effect of the electric field. Neither the effect of current nor the extent of the rapid Joule-heating to the suspending medium shows a direct correlation with the observed hemolysis. Voltage pulsation of the erythrocyte suspension can induce a transmembrane potential across the cell membrane and, at a critical point, it either opens up or creates pores in the red cells. In isotonic saline the pores are small. They allow passage of potassium and sodium ions but not sucrose and hemoglobin molecules. The pores are larger in low ionic conditions, and permit permeation of sucrose molecules, but under no circumstances can hemoglobin leak out as the direct result of the voltage pulse. Kinetic measurements indicate that the hemolysis of the red cells follows a stepwise mechanism: leakage of ions leads to an osmotic imbalance which in turn causes a colloidal hemolysis of the red cells. Other effects of the voltage pulsation are also discussed.",
author = "K. Kinosita and Tsong, {T. Y.}",
year = "1977",
language = "English",
volume = "74",
pages = "1923--1927",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "5",

}

TY - JOUR

T1 - Hemolysis of human erythrocytes by a transient electric field

AU - Kinosita, K.

AU - Tsong, T. Y.

PY - 1977

Y1 - 1977

N2 - Exposure of human erythrocytes, under isotonic conditions, to a high voltage pulse of a few kV/cm leads to total hemolysis of the red cells. Experiments described herein demonstrate that the hemolysis is due to the effect of the electric field. Neither the effect of current nor the extent of the rapid Joule-heating to the suspending medium shows a direct correlation with the observed hemolysis. Voltage pulsation of the erythrocyte suspension can induce a transmembrane potential across the cell membrane and, at a critical point, it either opens up or creates pores in the red cells. In isotonic saline the pores are small. They allow passage of potassium and sodium ions but not sucrose and hemoglobin molecules. The pores are larger in low ionic conditions, and permit permeation of sucrose molecules, but under no circumstances can hemoglobin leak out as the direct result of the voltage pulse. Kinetic measurements indicate that the hemolysis of the red cells follows a stepwise mechanism: leakage of ions leads to an osmotic imbalance which in turn causes a colloidal hemolysis of the red cells. Other effects of the voltage pulsation are also discussed.

AB - Exposure of human erythrocytes, under isotonic conditions, to a high voltage pulse of a few kV/cm leads to total hemolysis of the red cells. Experiments described herein demonstrate that the hemolysis is due to the effect of the electric field. Neither the effect of current nor the extent of the rapid Joule-heating to the suspending medium shows a direct correlation with the observed hemolysis. Voltage pulsation of the erythrocyte suspension can induce a transmembrane potential across the cell membrane and, at a critical point, it either opens up or creates pores in the red cells. In isotonic saline the pores are small. They allow passage of potassium and sodium ions but not sucrose and hemoglobin molecules. The pores are larger in low ionic conditions, and permit permeation of sucrose molecules, but under no circumstances can hemoglobin leak out as the direct result of the voltage pulse. Kinetic measurements indicate that the hemolysis of the red cells follows a stepwise mechanism: leakage of ions leads to an osmotic imbalance which in turn causes a colloidal hemolysis of the red cells. Other effects of the voltage pulsation are also discussed.

UR - http://www.scopus.com/inward/record.url?scp=0042663396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042663396&partnerID=8YFLogxK

M3 - Article

C2 - 266714

AN - SCOPUS:0042663396

VL - 74

SP - 1923

EP - 1927

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 5

ER -