Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo

Yosuke Okamura, Shinji Takeoka, Yuji Teramura, Hitomi Maruyama, Eishun Tsuchida, Makoto Handa, Yasuo Ikeda

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    BACKGROUND: Prototypes of platelet (PLT) substitutes have been studied and the focus was on a dodecapeptide, HHLGGAKQAGDV (H12), which is a fibrinogen γ-chain carboxy-terminal sequence (γ 400-411) and exists only in the fibrinogen domain. STUDY DESIGN AND METHODS: H12 was conjugated to the surface of polymerized albumin particles (polyAlb) as biocompatible and biodegradable particles with a mean diameter of 260 ± 60 nm, and the hemostatic ability of H12-conjugated polyAlb (H12-polyAlb) under flow conditions and thrombocytopenic rats have been studied. RESULTS: H12-polyAlb enhanced the in vitro thrombus formation of activated PLTs on a collagen-immobilized plate when exposed to the flowing thrombocytopenic imitation blood. Furthermore, the analysis of the tail bleeding time of rats that were made thrombocytopenic by busulfan injection showed that H12-polyAlb had a hemostatic effect. Based on the bleeding time and the amount injected, the hemostatic capacity of 20 H12-polyAlb was estimated to correspond to that of one PLT. CONCLUSION: These results were important first steps toward the development of PLT substitutes and indicated that H12-polyAlb may be a suitable candidate for an alternative to human PLT concentrates transfused into thrombocytopenic patients in the future.

    Original languageEnglish
    Pages (from-to)1221-1228
    Number of pages8
    JournalTransfusion
    Volume45
    Issue number7
    DOIs
    Publication statusPublished - 2005 Jul

    Fingerprint

    Hemostatics
    Fibrinogen
    Albumins
    Blood Platelets
    Bleeding Time
    Busulfan
    Tail
    Thrombosis
    Collagen
    Injections
    In Vitro Techniques

    ASJC Scopus subject areas

    • Hematology
    • Immunology

    Cite this

    Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo. / Okamura, Yosuke; Takeoka, Shinji; Teramura, Yuji; Maruyama, Hitomi; Tsuchida, Eishun; Handa, Makoto; Ikeda, Yasuo.

    In: Transfusion, Vol. 45, No. 7, 07.2005, p. 1221-1228.

    Research output: Contribution to journalArticle

    Okamura, Yosuke ; Takeoka, Shinji ; Teramura, Yuji ; Maruyama, Hitomi ; Tsuchida, Eishun ; Handa, Makoto ; Ikeda, Yasuo. / Hemostatic effects of fibrinogen γ-chain dodecapeptide-conjugated polymerized albumin particles in vitro and in vivo. In: Transfusion. 2005 ; Vol. 45, No. 7. pp. 1221-1228.
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    AU - Takeoka, Shinji

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    AU - Maruyama, Hitomi

    AU - Tsuchida, Eishun

    AU - Handa, Makoto

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