Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice

Yuji Teramura, Yosuke Okamura, Shinji Takeoka, Hiromi Tsuchiyama, Hideki Narumi, Mie Kainoh, Makoto Handa, Yasuo Ikeda, Eishun Tsuchida

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.

    Original languageEnglish
    Pages (from-to)256-260
    Number of pages5
    JournalBiochemical and Biophysical Research Communications
    Volume306
    Issue number1
    DOIs
    Publication statusPublished - 2003 Jun 20

    Fingerprint

    Bearings (structural)
    Hemostatics
    Albumins
    Platelets
    Blood Platelets
    Integrin alpha2
    Integrin alpha2beta1
    Platelet Membrane Glycoproteins
    Injections
    Bleeding Time
    Hemostasis
    Intravenous Administration
    Collagen
    Control Groups

    Keywords

    • Bleeding time
    • Collagen
    • GPIa/IIa
    • Platelet substitutes
    • Platelets
    • Polymerized albumin particles
    • Thrombocytopenic mice

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Molecular Biology

    Cite this

    Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice. / Teramura, Yuji; Okamura, Yosuke; Takeoka, Shinji; Tsuchiyama, Hiromi; Narumi, Hideki; Kainoh, Mie; Handa, Makoto; Ikeda, Yasuo; Tsuchida, Eishun.

    In: Biochemical and Biophysical Research Communications, Vol. 306, No. 1, 20.06.2003, p. 256-260.

    Research output: Contribution to journalArticle

    Teramura, Y, Okamura, Y, Takeoka, S, Tsuchiyama, H, Narumi, H, Kainoh, M, Handa, M, Ikeda, Y & Tsuchida, E 2003, 'Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice', Biochemical and Biophysical Research Communications, vol. 306, no. 1, pp. 256-260. https://doi.org/10.1016/S0006-291X(03)00943-4
    Teramura, Yuji ; Okamura, Yosuke ; Takeoka, Shinji ; Tsuchiyama, Hiromi ; Narumi, Hideki ; Kainoh, Mie ; Handa, Makoto ; Ikeda, Yasuo ; Tsuchida, Eishun. / Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 306, No. 1. pp. 256-260.
    @article{1ec750d1c94441bda113efe5f0ff46cf,
    title = "Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice",
    abstract = "The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.",
    keywords = "Bleeding time, Collagen, GPIa/IIa, Platelet substitutes, Platelets, Polymerized albumin particles, Thrombocytopenic mice",
    author = "Yuji Teramura and Yosuke Okamura and Shinji Takeoka and Hiromi Tsuchiyama and Hideki Narumi and Mie Kainoh and Makoto Handa and Yasuo Ikeda and Eishun Tsuchida",
    year = "2003",
    month = "6",
    day = "20",
    doi = "10.1016/S0006-291X(03)00943-4",
    language = "English",
    volume = "306",
    pages = "256--260",
    journal = "Biochemical and Biophysical Research Communications",
    issn = "0006-291X",
    publisher = "Academic Press Inc.",
    number = "1",

    }

    TY - JOUR

    T1 - Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice

    AU - Teramura, Yuji

    AU - Okamura, Yosuke

    AU - Takeoka, Shinji

    AU - Tsuchiyama, Hiromi

    AU - Narumi, Hideki

    AU - Kainoh, Mie

    AU - Handa, Makoto

    AU - Ikeda, Yasuo

    AU - Tsuchida, Eishun

    PY - 2003/6/20

    Y1 - 2003/6/20

    N2 - The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.

    AB - The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.

    KW - Bleeding time

    KW - Collagen

    KW - GPIa/IIa

    KW - Platelet substitutes

    KW - Platelets

    KW - Polymerized albumin particles

    KW - Thrombocytopenic mice

    UR - http://www.scopus.com/inward/record.url?scp=0038730717&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0038730717&partnerID=8YFLogxK

    U2 - 10.1016/S0006-291X(03)00943-4

    DO - 10.1016/S0006-291X(03)00943-4

    M3 - Article

    C2 - 12788097

    AN - SCOPUS:0038730717

    VL - 306

    SP - 256

    EP - 260

    JO - Biochemical and Biophysical Research Communications

    JF - Biochemical and Biophysical Research Communications

    SN - 0006-291X

    IS - 1

    ER -