Hepatic confinement of newly produced erythrocytes caused by low-temperature exposure in Xenopus laevis

Shun Maekawa, Hitomi Iemura, Yuko Kuramochi, Nami Nogawa-Kosaka, Hironori Nishikawa, Takehito Okui, Youichi Aizawa, Takashi Kato

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Diminished erythrocyte count and erythropoiesis have been reported during hypothermia in some ectothermic animals. In this study, the African clawed frog, Xenopus laevis, was used to investigate the cause of hypothermia-induced anemia. We developed a new model of hypothermia at 5°C and monitored blood cell count and erythropoiesis on several days. Erythrocyte count declined by 30% on the first day following cold exposure (5°C) and mRNA expression of hemeoxygenase-1 was enhanced 10-fold; accumulation of iron as a result of heme degradation was observed in the liver. One day after low-temperature exposure, erythropoietin mRNA expression was elevated in the liver and lung compared with that at normal temperature (22°C) by qRT-PCR analysis. Examination of liver sections (i.e. the erythropoietic organ) showed an increase in odianisidine-positive erythrocytes in the hepatic sinusoid 5 days after the onset of low-temperature exposure compared with normal liver. Peripheral erythrocyte count remained low, indicating that newly produced erythrocytes did not migrate from the liver to the circulation during hypothermia. In conclusion, this study reveals hypothermic anemia as being associated with hepatic erythrocyte destruction; prolonged anemia during low-temperature exposure is concomitant with newly produced erythrocytes being confined to the liver and may lead to new insights into vertebrate hematopoiesis.

    Original languageEnglish
    Pages (from-to)3087-3095
    Number of pages9
    JournalJournal of Experimental Biology
    Volume215
    Issue number17
    DOIs
    Publication statusPublished - 2012 Sep

    Fingerprint

    Xenopus laevis
    anemia
    erythrocytes
    Erythrocytes
    liver
    Temperature
    hypothermia
    Liver
    Erythrocyte Count
    Hypothermia
    temperature
    erythrocyte count
    Anemia
    Erythropoiesis
    erythropoiesis
    frog
    vertebrate
    Liver Circulation
    blood
    Induced Hypothermia

    Keywords

    • Anemia
    • Erythropoiesis
    • Hypothermia
    • Low temperature
    • Xenopus

    ASJC Scopus subject areas

    • Animal Science and Zoology
    • Ecology, Evolution, Behavior and Systematics
    • Molecular Biology
    • Physiology
    • Insect Science
    • Aquatic Science

    Cite this

    Hepatic confinement of newly produced erythrocytes caused by low-temperature exposure in Xenopus laevis. / Maekawa, Shun; Iemura, Hitomi; Kuramochi, Yuko; Nogawa-Kosaka, Nami; Nishikawa, Hironori; Okui, Takehito; Aizawa, Youichi; Kato, Takashi.

    In: Journal of Experimental Biology, Vol. 215, No. 17, 09.2012, p. 3087-3095.

    Research output: Contribution to journalArticle

    Maekawa, S, Iemura, H, Kuramochi, Y, Nogawa-Kosaka, N, Nishikawa, H, Okui, T, Aizawa, Y & Kato, T 2012, 'Hepatic confinement of newly produced erythrocytes caused by low-temperature exposure in Xenopus laevis', Journal of Experimental Biology, vol. 215, no. 17, pp. 3087-3095. https://doi.org/10.1242/jeb.072488
    Maekawa, Shun ; Iemura, Hitomi ; Kuramochi, Yuko ; Nogawa-Kosaka, Nami ; Nishikawa, Hironori ; Okui, Takehito ; Aizawa, Youichi ; Kato, Takashi. / Hepatic confinement of newly produced erythrocytes caused by low-temperature exposure in Xenopus laevis. In: Journal of Experimental Biology. 2012 ; Vol. 215, No. 17. pp. 3087-3095.
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