Hepatic gene expression profile of lipid metabolism in rats: Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression

Yoshikazu Higami, Tomoshi Tsuchiya, Takuya Chiba, Haruyoshi Yamaza, Izumi Muraoka, Megumi Hirose, Toshimitsu Komatsu, Isao Shimokawa

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We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.

Original languageEnglish
Pages (from-to)1099-1110
Number of pages12
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Issue number11
Publication statusPublished - 2006 Nov
Externally publishedYes


ASJC Scopus subject areas

  • Ageing

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