Hepatic gene expression profile of lipid metabolism in rats: Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression

Yoshikazu Higami, Tomoshi Tsuchiya, Takuya Chiba, Haruyoshi Yamaza, Izumi Muraoka, Megumi Hirose, Toshimitsu Komatsu, Isao Shimokawa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.

Original languageEnglish
Pages (from-to)1099-1110
Number of pages12
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume61
Issue number11
Publication statusPublished - 2006 Nov
Externally publishedYes

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Caloric Restriction
Somatomedins
Transcriptome
Lipid Metabolism
Growth Hormone
Liver
Xenobiotics
Gene Expression
Peroxisome Proliferator-Activated Receptors
Transgenes
Triglycerides
Fatty Acids
Lipids
Food
Enzymes
Serum

ASJC Scopus subject areas

  • Ageing

Cite this

Hepatic gene expression profile of lipid metabolism in rats : Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression. / Higami, Yoshikazu; Tsuchiya, Tomoshi; Chiba, Takuya; Yamaza, Haruyoshi; Muraoka, Izumi; Hirose, Megumi; Komatsu, Toshimitsu; Shimokawa, Isao.

In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 61, No. 11, 11.2006, p. 1099-1110.

Research output: Contribution to journalArticle

Higami, Yoshikazu ; Tsuchiya, Tomoshi ; Chiba, Takuya ; Yamaza, Haruyoshi ; Muraoka, Izumi ; Hirose, Megumi ; Komatsu, Toshimitsu ; Shimokawa, Isao. / Hepatic gene expression profile of lipid metabolism in rats : Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression. In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences. 2006 ; Vol. 61, No. 11. pp. 1099-1110.
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