Abstract
We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.
| Original language | English |
|---|---|
| Pages (from-to) | 1099-1110 |
| Number of pages | 12 |
| Journal | Journals of Gerontology - Series A Biological Sciences and Medical Sciences |
| Volume | 61 |
| Issue number | 11 |
| Publication status | Published - 2006 Nov |
| Externally published | Yes |
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Hepatic gene expression profile of lipid metabolism in rats : Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression. / Higami, Yoshikazu; Tsuchiya, Tomoshi; Chiba, Takuya; Yamaza, Haruyoshi; Muraoka, Izumi; Hirose, Megumi; Komatsu, Toshimitsu; Shimokawa, Isao.
In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 61, No. 11, 11.2006, p. 1099-1110.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hepatic gene expression profile of lipid metabolism in rats
T2 - Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression
AU - Higami, Yoshikazu
AU - Tsuchiya, Tomoshi
AU - Chiba, Takuya
AU - Yamaza, Haruyoshi
AU - Muraoka, Izumi
AU - Hirose, Megumi
AU - Komatsu, Toshimitsu
AU - Shimokawa, Isao
PY - 2006/11
Y1 - 2006/11
N2 - We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.
AB - We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.
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UR - http://www.scopus.com/inward/citedby.url?scp=33845945453&partnerID=8YFLogxK
M3 - Article
C2 - 17167150
AN - SCOPUS:33845945453
VL - 61
SP - 1099
EP - 1110
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
SN - 1079-5006
IS - 11
ER -