TY - JOUR
T1 - Hepatic gene expression profile of lipid metabolism in rats
T2 - Impact of caloric restriction and growth hormone/insulin-like growth factor-1 suppression
AU - Higami, Yoshikazu
AU - Tsuchiya, Tomoshi
AU - Chiba, Takuya
AU - Yamaza, Haruyoshi
AU - Muraoka, Izumi
AU - Hirose, Megumi
AU - Komatsu, Toshimitsu
AU - Shimokawa, Isao
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2006/11
Y1 - 2006/11
N2 - We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.
AB - We investigated the role of the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis on caloric restriction (CR) using male wild-type and transgenic homozygous dwarf rats bearing an antisense GH transgene and their Fl heterozygous progeny fed either ad libitum or subjected to 30% CR. CR predominantly altered expression of hepatic genes involved in the stress response, xenobiotic metabolism, and lipid metabolism. Most gene expressions involved in stress response and xenobiotic metabolism were regulated in a GH/IGF-1-dependent manner, and those involved in lipid metabolism were regulated in a GH/IGF-1-independent manner. Moreover, CR enhanced the gene expression involved in fatty acid synthesis after feeding and those encoding mitochondrial β-oxidation enzymes during food shortage, probably via transcriptional regulation by peroxisome proliferator-activated receptor α. These results, taken together with serum biochemical measures and hepatic triglyceride content, suggest that CR promotes lipid utilization through hepatic transcriptional alteration and prevents hepatic steatosis in a GH/IGF-1-independent manner.
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U2 - 10.1093/gerona/61.11.1099
DO - 10.1093/gerona/61.11.1099
M3 - Article
C2 - 17167150
AN - SCOPUS:33845945453
VL - 61
SP - 1099
EP - 1110
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
SN - 1079-5006
IS - 11
ER -