Heterogeneity of serum and amniotic fluid prolactin in humans

H. Fukuoka, R. Hamamoto, M. Higurashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The molecular heterogeneity of PRL was studied in serum and amniotic fluid using immunoperoxidase electrophoresis. In the amniotic fluid, larger molecular variants (> 117 kD), small PRL (20 and 23 kD) and cleaved PRL were present in the nonreduced condition. Newly, three mercaptoethanol (MCE)-resistant forms (76, 64 and 53 kD), and 25- and 23-kD forms appeared after treatment with MCE. Two variants, 64 and 23 kD, did not bind to concanavalin A (Con A), indicating a simple peptide without sugars. Other glycosylated forms, 76, 53 and 25 kD, bound to ConA. In maternal serum, the same components as those in the amniotic fluid were seen, except for a scanty amount of 23 kD and a newly appearing glycosylated variant of 28 kD in the reduced condition. This 23-kD form also appeared with the administration of TRH in women.

Original languageEnglish
Pages (from-to)58-63
Number of pages6
JournalHormone Research
Volume35
Issue numberSUPPL. 1
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Amniotic Fluid
Prolactin
Mercaptoethanol
Serum
Concanavalin A
Electrophoresis
Mothers
Peptides
Therapeutics

Keywords

  • glycosylated prolactin
  • immunoperoxidase electrophoresis
  • prolactin heterogeneity

ASJC Scopus subject areas

  • Endocrinology

Cite this

Fukuoka, H., Hamamoto, R., & Higurashi, M. (1991). Heterogeneity of serum and amniotic fluid prolactin in humans. Hormone Research, 35(SUPPL. 1), 58-63.

Heterogeneity of serum and amniotic fluid prolactin in humans. / Fukuoka, H.; Hamamoto, R.; Higurashi, M.

In: Hormone Research, Vol. 35, No. SUPPL. 1, 1991, p. 58-63.

Research output: Contribution to journalArticle

Fukuoka, H, Hamamoto, R & Higurashi, M 1991, 'Heterogeneity of serum and amniotic fluid prolactin in humans', Hormone Research, vol. 35, no. SUPPL. 1, pp. 58-63.
Fukuoka H, Hamamoto R, Higurashi M. Heterogeneity of serum and amniotic fluid prolactin in humans. Hormone Research. 1991;35(SUPPL. 1):58-63.
Fukuoka, H. ; Hamamoto, R. ; Higurashi, M. / Heterogeneity of serum and amniotic fluid prolactin in humans. In: Hormone Research. 1991 ; Vol. 35, No. SUPPL. 1. pp. 58-63.
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