HIF-1α in myeloid cells promotes adipose tissue remodeling toward insulin resistance

Akiko Takikawa, Arshad Mahmood, Allah Nawaz, Tomonobu Kado, Keisuke Okabe, Seiji Yamamoto, Aminuddin Aminuddin, Satoko Senda, Koichi Tsuneyama, Masashi Ikutani, Yasuharu Watanabe, Yoshiko Igarashi, Yoshinori Nagai, Kiyoshi Takatsu, Keiichi Koizumi, Johji Imura, Nobuhito Goda, Masakiyo Sasahara, Michihiro Matsumoto, Kumiko SaekiTakashi Nakagawa, Shiho Fujisaka, Isao Usui, Kazuyuki Tobe

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Adipose tissue hypoxia is an important feature of pathological adipose tissue expansion. Hypoxia-inducible factor- 1a (HIF-1α) in adipocytes reportedly induces oxidative stress and fibrosis, rather than neoangiogenesis via vascular endothelial growth factor (VEGF)-A. We previously reported thatmacrophages in crown-like structures (CLSs) are both hypoxic and inflammatory. In the current study, we examined how macrophage HIF-1α is involved in high-fat diet (HFD)-induced inflammation, neovascularization, hypoxia, and insulin resistance using mice with myeloid cell-specific HIF-1α deletion that were fed an HFD. Myeloid cell-specific HIF-1a gene deletion protected against HFD-induced inflammation, CLS formation, poor vasculature development in the adipose tissue, and systemic insulin resistance. Despite a reduced expression of Vegfa in epididymal white adipose tissue (eWAT), the preadipocytes and endothelial cells of HIF-1α-deficient mice expressed higher levels of angiogenic factors, including Vegfa, Angpt1, Fgf1, and Fgf10 in accordance with preferable eWAT remodeling. Our in vitro study revealed that lipopolysaccharide-Treated bone marrow-derived macrophages directly inhibited the expression of angiogenic factors in 3T3-L1 preadipocytes. Thus, macrophage HIF- 1α is involved not only in the formation of CLSs, further enhancing the inflammatory responses, but also in the inhibition of neoangiogenesis in preadipocytes. We concluded that these two pathways contribute to the obesity-related physiology of pathological adipose tissue expansion, thus causing systemic insulin resistance.

Original languageEnglish
Pages (from-to)3649-3659
Number of pages11
JournalDiabetes
Volume65
Issue number12
DOIs
Publication statusPublished - 2016 Dec 1
Externally publishedYes

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Myeloid Cells
Insulin Resistance
Adipose Tissue
High Fat Diet
Crowns
Tissue Expansion
Cell Hypoxia
White Adipose Tissue
Angiogenesis Inducing Agents
Macrophages
Inflammation
Gene Deletion
Adipocytes
Vascular Endothelial Growth Factor A
Lipopolysaccharides
Oxidative Stress
Fibrosis
Endothelial Cells
Obesity
Hypoxia

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Takikawa, A., Mahmood, A., Nawaz, A., Kado, T., Okabe, K., Yamamoto, S., ... Tobe, K. (2016). HIF-1α in myeloid cells promotes adipose tissue remodeling toward insulin resistance. Diabetes, 65(12), 3649-3659. https://doi.org/10.2337/db16-0012

HIF-1α in myeloid cells promotes adipose tissue remodeling toward insulin resistance. / Takikawa, Akiko; Mahmood, Arshad; Nawaz, Allah; Kado, Tomonobu; Okabe, Keisuke; Yamamoto, Seiji; Aminuddin, Aminuddin; Senda, Satoko; Tsuneyama, Koichi; Ikutani, Masashi; Watanabe, Yasuharu; Igarashi, Yoshiko; Nagai, Yoshinori; Takatsu, Kiyoshi; Koizumi, Keiichi; Imura, Johji; Goda, Nobuhito; Sasahara, Masakiyo; Matsumoto, Michihiro; Saeki, Kumiko; Nakagawa, Takashi; Fujisaka, Shiho; Usui, Isao; Tobe, Kazuyuki.

In: Diabetes, Vol. 65, No. 12, 01.12.2016, p. 3649-3659.

Research output: Contribution to journalArticle

Takikawa, A, Mahmood, A, Nawaz, A, Kado, T, Okabe, K, Yamamoto, S, Aminuddin, A, Senda, S, Tsuneyama, K, Ikutani, M, Watanabe, Y, Igarashi, Y, Nagai, Y, Takatsu, K, Koizumi, K, Imura, J, Goda, N, Sasahara, M, Matsumoto, M, Saeki, K, Nakagawa, T, Fujisaka, S, Usui, I & Tobe, K 2016, 'HIF-1α in myeloid cells promotes adipose tissue remodeling toward insulin resistance', Diabetes, vol. 65, no. 12, pp. 3649-3659. https://doi.org/10.2337/db16-0012
Takikawa A, Mahmood A, Nawaz A, Kado T, Okabe K, Yamamoto S et al. HIF-1α in myeloid cells promotes adipose tissue remodeling toward insulin resistance. Diabetes. 2016 Dec 1;65(12):3649-3659. https://doi.org/10.2337/db16-0012
Takikawa, Akiko ; Mahmood, Arshad ; Nawaz, Allah ; Kado, Tomonobu ; Okabe, Keisuke ; Yamamoto, Seiji ; Aminuddin, Aminuddin ; Senda, Satoko ; Tsuneyama, Koichi ; Ikutani, Masashi ; Watanabe, Yasuharu ; Igarashi, Yoshiko ; Nagai, Yoshinori ; Takatsu, Kiyoshi ; Koizumi, Keiichi ; Imura, Johji ; Goda, Nobuhito ; Sasahara, Masakiyo ; Matsumoto, Michihiro ; Saeki, Kumiko ; Nakagawa, Takashi ; Fujisaka, Shiho ; Usui, Isao ; Tobe, Kazuyuki. / HIF-1α in myeloid cells promotes adipose tissue remodeling toward insulin resistance. In: Diabetes. 2016 ; Vol. 65, No. 12. pp. 3649-3659.
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AU - Mahmood, Arshad

AU - Nawaz, Allah

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AU - Okabe, Keisuke

AU - Yamamoto, Seiji

AU - Aminuddin, Aminuddin

AU - Senda, Satoko

AU - Tsuneyama, Koichi

AU - Ikutani, Masashi

AU - Watanabe, Yasuharu

AU - Igarashi, Yoshiko

AU - Nagai, Yoshinori

AU - Takatsu, Kiyoshi

AU - Koizumi, Keiichi

AU - Imura, Johji

AU - Goda, Nobuhito

AU - Sasahara, Masakiyo

AU - Matsumoto, Michihiro

AU - Saeki, Kumiko

AU - Nakagawa, Takashi

AU - Fujisaka, Shiho

AU - Usui, Isao

AU - Tobe, Kazuyuki

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