High-content analysis of single cells directly assembled on CMOS sensor based on color imaging

Tsuyoshi Tanaka, Tatsuya Saeki, Yoshihiko Sunaga, Tadashi Matsunaga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66. mm × 5.32. mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.

Original languageEnglish
Pages (from-to)1460-1465
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number4
DOIs
Publication statusPublished - 2010 Dec 15
Externally publishedYes

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Keywords

  • Chemiluminescent detection
  • Color imaging
  • Complementary metal oxide semiconductor (CMOS) sensor
  • High-content analysis
  • Single-cell analysis

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

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