High-content analysis of single cells directly assembled on CMOS sensor based on color imaging

Tsuyoshi Tanaka, Tatsuya Saeki, Yoshihiko Sunaga, Tadashi Matsunaga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66. mm × 5.32. mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.

Original languageEnglish
Pages (from-to)1460-1465
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number4
DOIs
Publication statusPublished - 2010 Dec 15
Externally publishedYes

Fingerprint

Single-Cell Analysis
Semiconductors
Oxides
Color
Metals
Imaging techniques
Sensors
Trypan Blue
Oxide semiconductors
HeLa Cells
Image sensors
Lenses
Light emitting diodes
Irradiation
Light

Keywords

  • Chemiluminescent detection
  • Color imaging
  • Complementary metal oxide semiconductor (CMOS) sensor
  • High-content analysis
  • Single-cell analysis

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Biotechnology
  • Electrochemistry

Cite this

High-content analysis of single cells directly assembled on CMOS sensor based on color imaging. / Tanaka, Tsuyoshi; Saeki, Tatsuya; Sunaga, Yoshihiko; Matsunaga, Tadashi.

In: Biosensors and Bioelectronics, Vol. 26, No. 4, 15.12.2010, p. 1460-1465.

Research output: Contribution to journalArticle

Tanaka, Tsuyoshi ; Saeki, Tatsuya ; Sunaga, Yoshihiko ; Matsunaga, Tadashi. / High-content analysis of single cells directly assembled on CMOS sensor based on color imaging. In: Biosensors and Bioelectronics. 2010 ; Vol. 26, No. 4. pp. 1460-1465.
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