Abstract
A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66. mm × 5.32. mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.
Original language | English |
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Pages (from-to) | 1460-1465 |
Number of pages | 6 |
Journal | Biosensors and Bioelectronics |
Volume | 26 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2010 Dec 15 |
Externally published | Yes |
Keywords
- Chemiluminescent detection
- Color imaging
- Complementary metal oxide semiconductor (CMOS) sensor
- High-content analysis
- Single-cell analysis
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Biotechnology
- Electrochemistry