Abstract
A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66. mm × 5.32. mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.
| Original language | English |
|---|---|
| Pages (from-to) | 1460-1465 |
| Number of pages | 6 |
| Journal | Biosensors and Bioelectronics |
| Volume | 26 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2010 Dec 15 |
| Externally published | Yes |
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Keywords
- Chemiluminescent detection
- Color imaging
- Complementary metal oxide semiconductor (CMOS) sensor
- High-content analysis
- Single-cell analysis
ASJC Scopus subject areas
- Biophysics
- Biomedical Engineering
- Biotechnology
- Electrochemistry
Cite this
High-content analysis of single cells directly assembled on CMOS sensor based on color imaging. / Tanaka, Tsuyoshi; Saeki, Tatsuya; Sunaga, Yoshihiko; Matsunaga, Tadashi.
In: Biosensors and Bioelectronics, Vol. 26, No. 4, 15.12.2010, p. 1460-1465.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-content analysis of single cells directly assembled on CMOS sensor based on color imaging
AU - Tanaka, Tsuyoshi
AU - Saeki, Tatsuya
AU - Sunaga, Yoshihiko
AU - Matsunaga, Tadashi
PY - 2010/12/15
Y1 - 2010/12/15
N2 - A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66. mm × 5.32. mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.
AB - A complementary metal oxide semiconductor (CMOS) image sensor was applied to high-content analysis of single cells which were assembled closely or directly onto the CMOS sensor surface. The direct assembling of cell groups on CMOS sensor surface allows large-field (6.66. mm × 5.32. mm in entire active area of CMOS sensor) imaging within a second. Trypan blue-stained and non-stained cells in the same field area on the CMOS sensor were successfully distinguished as white- and blue-colored images under white LED light irradiation. Furthermore, the chemiluminescent signals of each cell were successfully visualized as blue-colored images on CMOS sensor only when HeLa cells were placed directly on the micro-lens array of the CMOS sensor. Our proposed approach will be a promising technique for real-time and high-content analysis of single cells in a large-field area based on color imaging.
KW - Chemiluminescent detection
KW - Color imaging
KW - Complementary metal oxide semiconductor (CMOS) sensor
KW - High-content analysis
KW - Single-cell analysis
UR - http://www.scopus.com/inward/record.url?scp=78649724600&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78649724600&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2010.07.081
DO - 10.1016/j.bios.2010.07.081
M3 - Article
C2 - 20728336
AN - SCOPUS:78649724600
VL - 26
SP - 1460
EP - 1465
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 4
ER -