High-density microcavity array for cell detection

Single-cell analysis of hematopoietic stem cells in peripheral blood mononuclear cells

Masahito Hosokawa, Atsushi Arakaki, Masayuki Takahashi, Tetsushi Mori, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Detection and isolation of specific cell types from limited biological samples have become a major challenge in clinical diagnosis and cell biology research. Here, we report a high-density microcavity array for target cell detection in which thousands of single cells were neatly arrayed onto 10 000 microcavities with high efficiency at approximately 90% of the loaded cells. Cell-specific immunophenotypes were exclusively identified at the singlecell level by measuring fluorescence intensities of cells labeled with antibodies targeting cell surface markers, and the purity of hematopoietic stem cells (HSCs) within human peripheral blood analyzed by this system was correlated with those obtained by conventional flow cytometry. Furthermore, gene expression of the stem cell marker, CD34, was determined from HSCs by isolating single cells using a micromanipulator. This technology has proven to be an effective tool for target cell detection and subsequent cellular analytical research at the single-cell level.

Original languageEnglish
Pages (from-to)5308-5313
Number of pages6
JournalAnalytical Chemistry
Volume81
Issue number13
DOIs
Publication statusPublished - 2009 Jul 1
Externally publishedYes

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Microcavities
Stem cells
Blood
Cytology
Micromanipulators
Flow cytometry
Gene expression
Fluorescence
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

High-density microcavity array for cell detection : Single-cell analysis of hematopoietic stem cells in peripheral blood mononuclear cells. / Hosokawa, Masahito; Arakaki, Atsushi; Takahashi, Masayuki; Mori, Tetsushi; Takeyama, Haruko; Matsunaga, Tadashi.

In: Analytical Chemistry, Vol. 81, No. 13, 01.07.2009, p. 5308-5313.

Research output: Contribution to journalArticle

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