High-dimensional and large-scale phenotyping of yeast mutants

Yoshikazu Ohya; Jim Sese; Masashi Yukawa; Fumi Sano; Yoichiro Nakatani; Taro L. Saito; Ayaka Saka; Tomoyuki Fukuda; Satoru Ishihara; Satomi Oka; Genjiro Suzuki; Machika Watanabe; Aiko Hirata; Miwaka Ohtani; Hiroshi Sawai; Nicolas Fraysse; Jean Paul Latgé; Jean M. François; Markus Aebi; Seiji Tanaka; Sachiko Muramatsu; Hiroyuki Araki; Kintake Sonoike; Satoru Nogami; Shinichi Morishita

doi:10.1073/pnas.0509436102

High-dimensional and large-scale phenotyping of yeast mutants

Yoshikazu Ohya^*, Jim Sese, Masashi Yukawa, Fumi Sano, Yoichiro Nakatani, Taro L. Saito, Ayaka Saka, Tomoyuki Fukuda, Satoru Ishihara, Satomi Oka, Genjiro Suzuki, Machika Watanabe, Aiko Hirata, Miwaka Ohtani, Hiroshi Sawai, Nicolas Fraysse, Jean Paul Latgé, Jean M. François, Markus Aebi, Seiji TanakaSachiko Muramatsu, Hiroyuki Araki, Kintake Sonoike, Satoru Nogami, Shinichi Morishita

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

214 Citations (Scopus)

Abstract

One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.

Original language	English
Pages (from-to)	19015-19020
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	102
Issue number	52
DOIs	https://doi.org/10.1073/pnas.0509436102
Publication status	Published - 2005 Dec 27
Externally published	Yes

Keywords

Cell morphology
Functional genomics
High-dimensional phenotyping
Phenome

ASJC Scopus subject areas

General

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10.1073/pnas.0509436102

Cite this

Ohya, Y., Sese, J., Yukawa, M., Sano, F., Nakatani, Y., Saito, T. L., Saka, A., Fukuda, T., Ishihara, S., Oka, S., Suzuki, G., Watanabe, M., Hirata, A., Ohtani, M., Sawai, H., Fraysse, N., Latgé, J. P., François, J. M., Aebi, M., ... Morishita, S. (2005). High-dimensional and large-scale phenotyping of yeast mutants. Proceedings of the National Academy of Sciences of the United States of America, 102(52), 19015-19020. https://doi.org/10.1073/pnas.0509436102

Ohya, Y, Sese, J, Yukawa, M, Sano, F, Nakatani, Y, Saito, TL, Saka, A, Fukuda, T, Ishihara, S, Oka, S, Suzuki, G, Watanabe, M, Hirata, A, Ohtani, M, Sawai, H, Fraysse, N, Latgé, JP, François, JM, Aebi, M, Tanaka, S, Muramatsu, S, Araki, H, Sonoike, K, Nogami, S & Morishita, S 2005, 'High-dimensional and large-scale phenotyping of yeast mutants', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 52, pp. 19015-19020. https://doi.org/10.1073/pnas.0509436102

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AU - Ohya, Yoshikazu

AU - Sese, Jim

AU - Yukawa, Masashi

AU - Sano, Fumi

AU - Nakatani, Yoichiro

AU - Saito, Taro L.

AU - Saka, Ayaka

AU - Fukuda, Tomoyuki

AU - Ishihara, Satoru

AU - Oka, Satomi

AU - Suzuki, Genjiro

AU - Watanabe, Machika

AU - Hirata, Aiko

AU - Ohtani, Miwaka

AU - Sawai, Hiroshi

AU - Fraysse, Nicolas

AU - Latgé, Jean Paul

AU - François, Jean M.

AU - Aebi, Markus

AU - Tanaka, Seiji

AU - Muramatsu, Sachiko

AU - Araki, Hiroyuki

AU - Sonoike, Kintake

AU - Nogami, Satoru

AU - Morishita, Shinichi

PY - 2005/12/27

Y1 - 2005/12/27

N2 - One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.

AB - One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis of mutant traits. In this study, systematic and quantitative analyses of mutant traits are achieved in the budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis of fluorescent microscopic images of triple-stained cells makes it possible to treat morphological variations as quantitative traits. Deletion of nearly half of the yeast genes not essential for growth affects these morphological traits. Similar morphological phenotypes are caused by deletions of functionally related genes, enabling a functional assignment of a locus to a specific cellular pathway. The high-dimensional phenotypic analysis of defined yeast mutant strains provides another step toward attributing gene function to all of the genes in the yeast genome.

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KW - Functional genomics

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High-dimensional and large-scale phenotyping of yeast mutants

Abstract

Keywords

ASJC Scopus subject areas

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