High efficiency electrochemical immuno sensors using 3D comb electrodes

Nobuo Honda, Masashi Inaba, Takashi Katagiri, Shuichi Shoji, Hirotaka Sato, Takayuki Homma, Tetsuya Osaka, Mikiko Saito, Jun Mizuno, Yasuo Wada

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

To realize highly sensitive electrochemical immunoassays, a micro-fabricated three-dimensional (3D) electrode was fabricated and applied to enzyme immuno assay based on production of a redox species. The dimensions of the electrodes are 10 μm in width and 30 μm in height, with 20 μm spacing in between, and the 30 pairs of anode and cathode electrodes made up a single sensor. This structure lead to enhancement of the electrochemical reaction, nearly 100% of trap ratio of redox species. It can be applied to highly sensitive enzyme immuno sensing based on p-aminophenylphosphate (PAPP). Applicability of this technique to the immuno assay for one of the clinical diagnostic marker proteins (alpha-fetoprotein; AFP) from 6 to 500 ng/mL was demonstrated.

Original languageEnglish
Pages (from-to)2306-2309
Number of pages4
JournalBiosensors and Bioelectronics
Volume20
Issue number11
DOIs
Publication statusPublished - 2005 May 15

Keywords

  • 3D comb electrode
  • Amperometric immuno sensors
  • Electrochemical enzyme immunoassay

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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