High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat

Seine A. Shintani; Kotaro Oyama; Norio Fukuda; ShiN'Ichi Ishiwata

doi:10.1016/j.bbrc.2014.12.077

High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat

Seine A. Shintani, Kotaro Oyama, Norio Fukuda^*, ShiN'Ichi Ishiwata

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

In the present study, we investigated the effects of infra-red laser irradiation on sarcomere dynamics in living neonatal cardiomyocytes of the rat. A rapid increase in temperature to >∼38°C induced [Ca²⁺]_i-independent high-frequency (∼5-10 Hz) sarcomeric auto-oscillations (Hyperthermal Sarcomeric Oscillations; HSOs). In myocytes with the intact sarcoplasmic reticular functions, HSOs coexisted with [Ca²⁺]_i-dependent spontaneous beating in the same sarcomeres, with markedly varying frequencies (∼10 and ∼1 Hz for the former and latter, respectively). HSOs likewise occurred following blockade of the sarcoplasmic reticular functions, with the amplitude becoming larger and the frequency lower in a time-dependent manner. The present findings suggest that in the mammalian heart, sarcomeres spontaneously oscillate at higher frequencies than the sinus rhythm at temperatures slightly above the physiologically relevant levels.

Original language	English
Pages (from-to)	165-170
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	457
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2014.12.077
Publication status	Published - 2015 Feb 6

Keywords

Cardiac muscle
Contractility
Imaging
Sarcomere dynamics

ASJC Scopus subject areas

Biochemistry
Biophysics
Cell Biology
Molecular Biology

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10.1016/j.bbrc.2014.12.077

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title = "High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat",

abstract = "In the present study, we investigated the effects of infra-red laser irradiation on sarcomere dynamics in living neonatal cardiomyocytes of the rat. A rapid increase in temperature to >∼38°C induced [Ca2+]i-independent high-frequency (∼5-10 Hz) sarcomeric auto-oscillations (Hyperthermal Sarcomeric Oscillations; HSOs). In myocytes with the intact sarcoplasmic reticular functions, HSOs coexisted with [Ca2+]i-dependent spontaneous beating in the same sarcomeres, with markedly varying frequencies (∼10 and ∼1 Hz for the former and latter, respectively). HSOs likewise occurred following blockade of the sarcoplasmic reticular functions, with the amplitude becoming larger and the frequency lower in a time-dependent manner. The present findings suggest that in the mammalian heart, sarcomeres spontaneously oscillate at higher frequencies than the sinus rhythm at temperatures slightly above the physiologically relevant levels.",

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PY - 2015/2/6

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High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat

Abstract

Keywords

ASJC Scopus subject areas

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