High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat

Seine A. Shintani, Kotaro Oyama, Norio Fukuda, ShiN'Ichi Ishiwata

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    In the present study, we investigated the effects of infra-red laser irradiation on sarcomere dynamics in living neonatal cardiomyocytes of the rat. A rapid increase in temperature to >∼38°C induced [Ca2+]i-independent high-frequency (∼5-10 Hz) sarcomeric auto-oscillations (Hyperthermal Sarcomeric Oscillations; HSOs). In myocytes with the intact sarcoplasmic reticular functions, HSOs coexisted with [Ca2+]i-dependent spontaneous beating in the same sarcomeres, with markedly varying frequencies (∼10 and ∼1 Hz for the former and latter, respectively). HSOs likewise occurred following blockade of the sarcoplasmic reticular functions, with the amplitude becoming larger and the frequency lower in a time-dependent manner. The present findings suggest that in the mammalian heart, sarcomeres spontaneously oscillate at higher frequencies than the sinus rhythm at temperatures slightly above the physiologically relevant levels.

    Original languageEnglish
    Pages (from-to)165-170
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume457
    Issue number2
    DOIs
    Publication statusPublished - 2015 Feb 6

    Fingerprint

    Sarcomeres
    Cardiac Myocytes
    Heating
    Rats
    Infrared lasers
    Laser beam effects
    Temperature
    Muscle Cells
    Lasers

    Keywords

    • Cardiac muscle
    • Contractility
    • Imaging
    • Sarcomere dynamics

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Cell Biology
    • Molecular Biology

    Cite this

    High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat. / Shintani, Seine A.; Oyama, Kotaro; Fukuda, Norio; Ishiwata, ShiN'Ichi.

    In: Biochemical and Biophysical Research Communications, Vol. 457, No. 2, 06.02.2015, p. 165-170.

    Research output: Contribution to journalArticle

    Shintani, Seine A. ; Oyama, Kotaro ; Fukuda, Norio ; Ishiwata, ShiN'Ichi. / High-frequency sarcomeric auto-oscillations induced by heating in living neonatal cardiomyocytes of the rat. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 457, No. 2. pp. 165-170.
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