High performance electrophoretic analysis on cycloolefin polymer microchips

Fumihiko Kitagawa, Takafumi Suzuki, Jun Mizuno, Shuichi Shoji, Koji Otsuka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

Original languageEnglish
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherJapan Academic Association Inc
Pages362-364
Number of pages3
Publication statusPublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CityTokyo
Period06/11/506/11/9

Fingerprint

Electrophoresis
Polymers
Bovine Serum Albumin
Tryptophan
Amino acids
Lenses
Microscopes
Coloring Agents
Dyes
Proteins
Amino Acids
Hot Temperature

Keywords

  • Chiral separation
  • Microchip electrophoresis
  • Protein
  • Sweeping
  • Thermal lens microscope

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kitagawa, F., Suzuki, T., Mizuno, J., Shoji, S., & Otsuka, K. (2006). High performance electrophoretic analysis on cycloolefin polymer microchips. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 362-364). Japan Academic Association Inc.

High performance electrophoretic analysis on cycloolefin polymer microchips. / Kitagawa, Fumihiko; Suzuki, Takafumi; Mizuno, Jun; Shoji, Shuichi; Otsuka, Koji.

Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. p. 362-364.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kitagawa, F, Suzuki, T, Mizuno, J, Shoji, S & Otsuka, K 2006, High performance electrophoretic analysis on cycloolefin polymer microchips. in Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, pp. 362-364, 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, 06/11/5.
Kitagawa F, Suzuki T, Mizuno J, Shoji S, Otsuka K. High performance electrophoretic analysis on cycloolefin polymer microchips. In Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc. 2006. p. 362-364
Kitagawa, Fumihiko ; Suzuki, Takafumi ; Mizuno, Jun ; Shoji, Shuichi ; Otsuka, Koji. / High performance electrophoretic analysis on cycloolefin polymer microchips. Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Japan Academic Association Inc, 2006. pp. 362-364
@inproceedings{3d7521b8f2fa4fed890d1d1dc4aee075,
title = "High performance electrophoretic analysis on cycloolefin polymer microchips",
abstract = "This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.",
keywords = "Chiral separation, Microchip electrophoresis, Protein, Sweeping, Thermal lens microscope",
author = "Fumihiko Kitagawa and Takafumi Suzuki and Jun Mizuno and Shuichi Shoji and Koji Otsuka",
year = "2006",
language = "English",
pages = "362--364",
booktitle = "Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences",
publisher = "Japan Academic Association Inc",

}

TY - GEN

T1 - High performance electrophoretic analysis on cycloolefin polymer microchips

AU - Kitagawa, Fumihiko

AU - Suzuki, Takafumi

AU - Mizuno, Jun

AU - Shoji, Shuichi

AU - Otsuka, Koji

PY - 2006

Y1 - 2006

N2 - This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

AB - This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

KW - Chiral separation

KW - Microchip electrophoresis

KW - Protein

KW - Sweeping

KW - Thermal lens microscope

UR - http://www.scopus.com/inward/record.url?scp=84902523691&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902523691&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84902523691

SP - 362

EP - 364

BT - Micro Total Analysis Systems - Proceedings of MicroTAS 2006 Conference: 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Japan Academic Association Inc

ER -