High performance electrophoretic analysis on cycloolefin polymer microchips

Fumihiko Kitagawa, Takafumi Suzuki, Jun Mizuno, Shuichi Shoji, Koji Otsuka

Research output: Contribution to conferencePaper

Abstract

This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

Original languageEnglish
Pages362-364
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

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Keywords

  • Chiral separation
  • Microchip electrophoresis
  • Protein
  • Sweeping
  • Thermal lens microscope

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Kitagawa, F., Suzuki, T., Mizuno, J., Shoji, S., & Otsuka, K. (2006). High performance electrophoretic analysis on cycloolefin polymer microchips. 362-364. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.