High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Yoichi Murakami, Takahiro Arakawa, Sachiko Nomura, Yoshinori Yamaguchi, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    3 Citations (Scopus)

    Abstract

    In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

    Original languageEnglish
    Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
    PublisherChemical and Biological Microsystems Society
    Pages1774-1776
    Number of pages3
    Publication statusPublished - 2008
    Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
    Duration: 2008 Oct 122008 Oct 16

    Other

    Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
    CitySan Diego, CA
    Period08/10/1208/10/16

    Fingerprint

    Microfluidics
    Labeling
    Capillary electrophoresis
    Sodium dodecyl sulfate
    Sodium Dodecyl Sulfate

    Keywords

    • Cell labeling
    • Cell manipulation
    • Cell trapping
    • Single cell lysate extraction

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Murakami, Y., Arakawa, T., Nomura, S., Yamaguchi, Y., & Shoji, S. (2008). High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1774-1776). Chemical and Biological Microsystems Society.

    High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. / Murakami, Yoichi; Arakawa, Takahiro; Nomura, Sachiko; Yamaguchi, Yoshinori; Shoji, Shuichi.

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 1774-1776.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Murakami, Y, Arakawa, T, Nomura, S, Yamaguchi, Y & Shoji, S 2008, High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 1774-1776, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, 08/10/12.
    Murakami Y, Arakawa T, Nomura S, Yamaguchi Y, Shoji S. High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 1774-1776
    Murakami, Yoichi ; Arakawa, Takahiro ; Nomura, Sachiko ; Yamaguchi, Yoshinori ; Shoji, Shuichi. / High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 1774-1776
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