High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Yoichi Murakami, Takahiro Arakawa, Sachiko Nomura, Yoshinori Yamaguchi, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    3 Citations (Scopus)

    Abstract

    In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

    Original languageEnglish
    Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
    PublisherChemical and Biological Microsystems Society
    Pages1774-1776
    Number of pages3
    Publication statusPublished - 2008
    Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
    Duration: 2008 Oct 122008 Oct 16

    Other

    Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
    CitySan Diego, CA
    Period08/10/1208/10/16

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    Keywords

    • Cell labeling
    • Cell manipulation
    • Cell trapping
    • Single cell lysate extraction

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Murakami, Y., Arakawa, T., Nomura, S., Yamaguchi, Y., & Shoji, S. (2008). High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1774-1776). Chemical and Biological Microsystems Society.