High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Yoichi Murakami, Takahiro Arakawa, Sachiko Nomura, Yoshinori Yamaguchi, Shuichi Shoji

Research output: Contribution to conferencePaper

3 Citations (Scopus)

Abstract

In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

Original languageEnglish
Pages1774-1776
Number of pages3
Publication statusPublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Cell labeling
  • Cell manipulation
  • Cell trapping
  • Single cell lysate extraction

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Murakami, Y., Arakawa, T., Nomura, S., Yamaguchi, Y., & Shoji, S. (2008). High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. 1774-1776. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.