High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection

Kazuto Ozaki, Hirokazu Sugino, Takahiro Arakawa, Yoshitaka Shirasaki, Takashi Funatsu, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Three kinds of Escherichia coli (E. coli) cells, labeled with different fluorescent proteins (GFP, Phi-Yellow, or DsRed), were successfully separated with the recovery ratio of more than 80% and the purity of more than 90% in a multiple sorting system. In the case of GFP labeled E. coli cells, 99.8±0.3% in purity was achieved and more than 1000 E.coli cells were separated within 60 min.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1856-1858
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Keywords

  • Biomolecule sorter
  • E. coli
  • Hydrogel
  • Multi sorting

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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