TY - GEN
T1 - High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection
AU - Ozaki, Kazuto
AU - Sugino, Hirokazu
AU - Arakawa, Takahiro
AU - Shirasaki, Yoshitaka
AU - Funatsu, Takashi
AU - Shoji, Shuichi
PY - 2009
Y1 - 2009
N2 - Three kinds of Escherichia coli (E. coli) cells, labeled with different fluorescent proteins (GFP, Phi-Yellow, or DsRed), were successfully separated with the recovery ratio of more than 80% and the purity of more than 90% in a multiple sorting system. In the case of GFP labeled E. coli cells, 99.8±0.3% in purity was achieved and more than 1000 E.coli cells were separated within 60 min.
AB - Three kinds of Escherichia coli (E. coli) cells, labeled with different fluorescent proteins (GFP, Phi-Yellow, or DsRed), were successfully separated with the recovery ratio of more than 80% and the purity of more than 90% in a multiple sorting system. In the case of GFP labeled E. coli cells, 99.8±0.3% in purity was achieved and more than 1000 E.coli cells were separated within 60 min.
KW - Biomolecule sorter
KW - E. coli
KW - Hydrogel
KW - Multi sorting
UR - http://www.scopus.com/inward/record.url?scp=80052289671&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052289671&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:80052289671
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 1856
EP - 1858
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -