High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection

Kazuto Ozaki, Hirokazu Sugino, Takahiro Arakawa, Yoshitaka Shirasaki, Takashi Funatsu, Shuichi Shoji

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    Three kinds of Escherichia coli (E. coli) cells, labeled with different fluorescent proteins (GFP, Phi-Yellow, or DsRed), were successfully separated with the recovery ratio of more than 80% and the purity of more than 90% in a multiple sorting system. In the case of GFP labeled E. coli cells, 99.8±0.3% in purity was achieved and more than 1000 E.coli cells were separated within 60 min.

    Original languageEnglish
    Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Pages1856-1858
    Number of pages3
    ISBN (Print)9780979806421
    Publication statusPublished - 2009
    Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju
    Duration: 2009 Nov 12009 Nov 5

    Other

    Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
    CityJeju
    Period09/11/109/11/5

    Fingerprint

    Hydrogel
    Sorting
    Hydrogels
    Escherichia coli
    Fluorescence
    Cells
    Proteins
    Recovery

    Keywords

    • Biomolecule sorter
    • E. coli
    • Hydrogel
    • Multi sorting

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Ozaki, K., Sugino, H., Arakawa, T., Shirasaki, Y., Funatsu, T., & Shoji, S. (2009). High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1856-1858). Chemical and Biological Microsystems Society.

    High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection. / Ozaki, Kazuto; Sugino, Hirokazu; Arakawa, Takahiro; Shirasaki, Yoshitaka; Funatsu, Takashi; Shoji, Shuichi.

    Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 1856-1858.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Ozaki, K, Sugino, H, Arakawa, T, Shirasaki, Y, Funatsu, T & Shoji, S 2009, High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 1856-1858, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, 09/11/1.
    Ozaki K, Sugino H, Arakawa T, Shirasaki Y, Funatsu T, Shoji S. High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 1856-1858
    Ozaki, Kazuto ; Sugino, Hirokazu ; Arakawa, Takahiro ; Shirasaki, Yoshitaka ; Funatsu, Takashi ; Shoji, Shuichi. / High performance multiple E. coli cell sorting system using thrmosensitive hydrogel and fluorescence spectrum detection. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 1856-1858
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