High performance parallel bioparticle sorter with 3-dimensional PDMS chip

H. Sugino, Y. Nara, Y. Shirasaki, T. Arakawa, Shuichi Shoji, T. Funatsu

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    This paper reports an improved method for achieving on-chip parallel sorting of fluorescently labeled bioparticles. We have integrated 8-parallel sheath flow sorters, in which a sample stream is sheathed by two buffer streams to focus the sample stream, into a single chip. Each sorter integrated in the chip successfully sorted fluorescent microspheres and Escherichia coli (E. coli) cells expressing fluorescent proteins with 10-20 ms of flow switching time and an 85-95% recovery ratio.

    Original languageEnglish
    Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
    PublisherChemical and Biological Microsystems Society
    Pages1184-1186
    Number of pages3
    Publication statusPublished - 2008
    Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA
    Duration: 2008 Oct 122008 Oct 16

    Other

    Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
    CitySan Diego, CA
    Period08/10/1208/10/16

    Fingerprint

    Microspheres
    Sorting
    Escherichia coli
    Buffers
    Cells
    Proteins
    Recovery

    Keywords

    • Microfluidics
    • Multi-layer PDMS
    • Parallel sorting
    • Sol-gel transition

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

    Cite this

    Sugino, H., Nara, Y., Shirasaki, Y., Arakawa, T., Shoji, S., & Funatsu, T. (2008). High performance parallel bioparticle sorter with 3-dimensional PDMS chip. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1184-1186). Chemical and Biological Microsystems Society.

    High performance parallel bioparticle sorter with 3-dimensional PDMS chip. / Sugino, H.; Nara, Y.; Shirasaki, Y.; Arakawa, T.; Shoji, Shuichi; Funatsu, T.

    12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 1184-1186.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Sugino, H, Nara, Y, Shirasaki, Y, Arakawa, T, Shoji, S & Funatsu, T 2008, High performance parallel bioparticle sorter with 3-dimensional PDMS chip. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 1184-1186, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, 08/10/12.
    Sugino H, Nara Y, Shirasaki Y, Arakawa T, Shoji S, Funatsu T. High performance parallel bioparticle sorter with 3-dimensional PDMS chip. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society. 2008. p. 1184-1186
    Sugino, H. ; Nara, Y. ; Shirasaki, Y. ; Arakawa, T. ; Shoji, Shuichi ; Funatsu, T. / High performance parallel bioparticle sorter with 3-dimensional PDMS chip. 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. pp. 1184-1186
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