High performance parallel bioparticle sorter with 3-dimensional PDMS chip

H. Sugino, Y. Nara, Y. Shirasaki, T. Arakawa, S. Shoji, T. Funatsu

Research output: Contribution to conferencePaper

Abstract

This paper reports an improved method for achieving on-chip parallel sorting of fluorescently labeled bioparticles. We have integrated 8-parallel sheath flow sorters, in which a sample stream is sheathed by two buffer streams to focus the sample stream, into a single chip. Each sorter integrated in the chip successfully sorted fluorescent microspheres and Escherichia coli (E. coli) cells expressing fluorescent proteins with 10-20 ms of flow switching time and an 85-95% recovery ratio.

Original languageEnglish
Pages1184-1186
Number of pages3
Publication statusPublished - 2008 Jan 1
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: 2008 Oct 122008 Oct 16

Conference

Conference12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period08/10/1208/10/16

Keywords

  • Microfluidics
  • Multi-layer PDMS
  • Parallel sorting
  • Sol-gel transition

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Sugino, H., Nara, Y., Shirasaki, Y., Arakawa, T., Shoji, S., & Funatsu, T. (2008). High performance parallel bioparticle sorter with 3-dimensional PDMS chip. 1184-1186. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.