High-precision multiclass cell classification by supervised machine learning on lectin microarray data

Mayu Shibata, Kohji Okamura, Kei Yura, Akihiro Umezawa

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Establishment of a cell classification platform for evaluation and selection of human pluripotent stem cells (hPSCs) is of great importance to assure the efficacy and safety of cell-based therapy. In our previous work, we introduced a discriminant function that evaluates pluripotency from the cells’ glycome. However, it is not yet suitable for general use. Methods: The current study aims to establish a high-precision cell classification platform introducing supervised machine learning and test the platform on glycome analysis as a proof-of-concept study. We employed linear classification and neural network to the lectin microarray data from 1577 human cells and categorized them into five classes including hPSCs. Results: The linear-classification-based model and the neural-network-based model successfully predicted the sample type with accuracies of 89% and 97%, respectively. Conclusions: Because of the high recognition accuracies and the small amount of computing resources required for these analyses, our platform can be a high precision conventional cell classification system for hPSCs.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalRegenerative Therapy
Volume15
DOIs
Publication statusPublished - 2020 Dec

Keywords

  • Artificial intelligence
  • Lectin microarray
  • Linear classification
  • Neural network
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Developmental Biology

Fingerprint Dive into the research topics of 'High-precision multiclass cell classification by supervised machine learning on lectin microarray data'. Together they form a unique fingerprint.

Cite this