High speed digital protein interaction analysis using microfluidic single molecule detection system

Chao Kai Chou, Nan Jing, Hirohito Yamaguchi, Pei Hsiang Tsou, Heng Huan Lee, Chun Te Chen, Ying Nai Wang, Sungmin Hong, Chin Su, Jun Kameoka*, Mien Chie Hung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The understanding of protein interaction dynamics is important for signal transduction research but current available techniques prove difficult in addressing this issue. Thus, using the microfluidic approach, we developed a digital protein analytical platform and methodology named MAPS (Microfluidic system Analyzing Protein in Single complex) that can measure the amount of target proteins and protein complexes at the digitally single molecule resolution. By counting protein events individually, this system can provide rough protein interaction ratios which will be critical for understanding signal transduction dynamics. In addition, this system only requires less than an hour to characterize the target protein sample, which is much quicker than conventional approaches. As a proof of concept, we have determined the interaction ratios of oncogenic signaling protein complexes EGFR/Src and EGFR/STAT3 before and after EGF ligand stimulation. To the best of our knowledge, this is the first time that the interaction ratio between EGFR and its downstream proteins has been characterized. The information from MAPS will be critical for the study of protein signal transduction quantitation and dynamics.

Original languageEnglish
Pages (from-to)1793-1798
Number of pages6
JournalLab on a Chip
Volume10
Issue number14
DOIs
Publication statusPublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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