High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow

Takahiro Arakawa; Toshimitsu Izumi; Wataru Teragauchi; Tokihiko Aoki; Yoshitaka Shirasaki; Hirokazu Sugino; Takashi Funatsu; Shuichi Shoji

High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow

Takahiro Arakawa^*, Toshimitsu Izumi, Wataru Teragauchi, Tokihiko Aoki, Yoshitaka Shirasaki, Hirokazu Sugino, Takashi Funatsu, Shuichi Shoji

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

High speed particles and biomolecules sorter was realized using thermosensitive hydrogel and water two-phase sheath flow system. The two phase sheath flow consists of a water flow including samples and two hydrogel carrier flows is utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorter. Flow switching is performed by the sol-gel transfer of the thermosensitive hydrogel generated by the focused IR laser irradiation. High speed sorting less than 5.0 msec and no error sorting (3000 counts) was realized. Since sorting performed in simple PDMS-glass microchannels without any electric stimulation, the proposed system is suitable for bio-applications.

Original language	English
Title of host publication	19th IEEE International Conference on Micro Electro Mechanical Systems
Pages	58-61
Number of pages	4
Publication status	Published - 2006 Oct 24
Externally published	Yes
Event	19th IEEE International Conference on Micro Electro Mechanical Systems - Istanbul, Turkey Duration: 2006 Jan 22 → 2006 Jan 26

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume	2006
ISSN (Print)	1084-6999

Conference

Conference	19th IEEE International Conference on Micro Electro Mechanical Systems
Country/Territory	Turkey
City	Istanbul
Period	06/1/22 → 06/1/26

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Cite this

Arakawa, T., Izumi, T., Teragauchi, W., Aoki, T., Shirasaki, Y., Sugino, H., Funatsu, T., & Shoji, S. (2006). High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow. In 19th IEEE International Conference on Micro Electro Mechanical Systems (pp. 58-61). [1627735] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).

High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow. / Arakawa, Takahiro; Izumi, Toshimitsu; Teragauchi, Wataru et al.

19th IEEE International Conference on Micro Electro Mechanical Systems. 2006. p. 58-61 1627735 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2006).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Arakawa, T, Izumi, T, Teragauchi, W, Aoki, T, Shirasaki, Y, Sugino, H, Funatsu, T & Shoji, S 2006, High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow. in 19th IEEE International Conference on Micro Electro Mechanical Systems., 1627735, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), vol. 2006, pp. 58-61, 19th IEEE International Conference on Micro Electro Mechanical Systems, Istanbul, Turkey, 06/1/22.

Arakawa T, Izumi T, Teragauchi W, Aoki T, Shirasaki Y, Sugino H et al. High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow. In 19th IEEE International Conference on Micro Electro Mechanical Systems. 2006. p. 58-61. 1627735. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "High speed particles and biomolecules sorter was realized using thermosensitive hydrogel and water two-phase sheath flow system. The two phase sheath flow consists of a water flow including samples and two hydrogel carrier flows is utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorter. Flow switching is performed by the sol-gel transfer of the thermosensitive hydrogel generated by the focused IR laser irradiation. High speed sorting less than 5.0 msec and no error sorting (3000 counts) was realized. Since sorting performed in simple PDMS-glass microchannels without any electric stimulation, the proposed system is suitable for bio-applications.",

author = "Takahiro Arakawa and Toshimitsu Izumi and Wataru Teragauchi and Tokihiko Aoki and Yoshitaka Shirasaki and Hirokazu Sugino and Takashi Funatsu and Shuichi Shoji",

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AU - Arakawa, Takahiro

AU - Izumi, Toshimitsu

AU - Teragauchi, Wataru

AU - Aoki, Tokihiko

AU - Shirasaki, Yoshitaka

AU - Sugino, Hirokazu

AU - Funatsu, Takashi

AU - Shoji, Shuichi

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N2 - High speed particles and biomolecules sorter was realized using thermosensitive hydrogel and water two-phase sheath flow system. The two phase sheath flow consists of a water flow including samples and two hydrogel carrier flows is utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorter. Flow switching is performed by the sol-gel transfer of the thermosensitive hydrogel generated by the focused IR laser irradiation. High speed sorting less than 5.0 msec and no error sorting (3000 counts) was realized. Since sorting performed in simple PDMS-glass microchannels without any electric stimulation, the proposed system is suitable for bio-applications.

AB - High speed particles and biomolecules sorter was realized using thermosensitive hydrogel and water two-phase sheath flow system. The two phase sheath flow consists of a water flow including samples and two hydrogel carrier flows is utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorter. Flow switching is performed by the sol-gel transfer of the thermosensitive hydrogel generated by the focused IR laser irradiation. High speed sorting less than 5.0 msec and no error sorting (3000 counts) was realized. Since sorting performed in simple PDMS-glass microchannels without any electric stimulation, the proposed system is suitable for bio-applications.

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High speed particles and biomolecules sorting microsystem using thermosensitive hydrogel and water two-phase flow

Abstract

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ASJC Scopus subject areas

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