High-speed particles and biomolecules sorting microsystem using thermosensitive hydrogel

Takahiro Arakawa, Yoshitaka Shirasaki, Toshimitsu Izumi, Tokihiko Aoki, Hirokazu Sugino, Takashi Funatsu, Shuichi Shoji

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two types of high-speed particle and biomolecule sheath flow sorters were realized using thermosensitive hydrogel. One is all hydrogel sheath flow and the other is two-phase sheath flow that consists of a water flow including samples and two hydrogel carrier flows; these have been utilized instead of the air and water droplet two-phase flow used in the conventional commercialized cell sorting system. Flow switching is performed by the sol-gel transfer of the thermosensitive hydrogel generated by focused infrared (IR) laser irradiation. High-speed sorting less than 5.0 ms and no error sorting (120 000 counts) in the all hydrogel sheath flow system while 20 ms in the two-phase sheath flow was realized. Since sorting was performed in simple PDMS-glass microchannels without any electric stimulation and mechanical valve structures, the proposed system is suitable for many biochemical applications.

Original languageEnglish
Article numberS04
Pages (from-to)3141-3146
Number of pages6
JournalMeasurement Science and Technology
Volume17
Issue number12
DOIs
Publication statusPublished - 2006 Dec 1

Keywords

  • Cell sorter
  • IR laser
  • PDMS microsystem
  • Particle and biomolecule sorter
  • Sheath flow
  • Thermosensitive hydrogel

ASJC Scopus subject areas

  • Instrumentation
  • Engineering (miscellaneous)
  • Applied Mathematics

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