High-throughput screening identifies artesunate as selective inhibitor of cancer stemness: Involvement of mitochondrial metabolism

Amit Subedi, Yushi Futamura, Mayuko Nishi, Akihide Ryo, Nobumoto Watanabe, Hiroyuki Osada*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Cancer stem cells (CSCs) have robust systems to maintain cancer stemness and drug resistance. Thus, targeting such robust systems instead of focusing on individual signaling pathways should be the approach allowing the identification of selective CSC inhibitors. Here, we used the alkaline phosphatase (ALP) assay to identify inhibitors for cancer stemness in induced cancer stem-like (iCSCL) cells. We screened several compounds from natural product chemical library and evaluated hit compounds for their efficacy on cancer stemness in iCSCL tumorspheres. We identified artesunate, an antimalarial drug, as a selective inhibitor of cancer stemness. Artesunate induced mitochondrial dysfunction that selectively inhibited cancer stemness of iCSCL cells, indicating an essential role of mitochondrial metabolism in cancer stemness.

Original languageEnglish
Pages (from-to)737-742
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume477
Issue number4
DOIs
Publication statusPublished - 2016 Sep 2
Externally publishedYes

Keywords

  • Artesunate
  • CSCs
  • High-throughput screening
  • Mitochondrial metabolism
  • Tumorspheres

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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