High-throughput single-cell secretion measurement on an optical wavegude chip

Y. Shirasaki, N. Suzuki, M. Yamagishi, A. Nakahara, Shuichi Shoji, O. Ohara

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    1 Citation (Scopus)

    Abstract

    We report a newly developed platform that enables us to monitor protein secretion from more than hundreds individual single cells in real time simultaneously. The platform was achieved after improvement of the limited size of view field in total internal reflection fluorescence microscopy, which was owing to using optical waveguide technology. Although the coupling of PDMS microwells and glass waveguides raised another problem regarding elevated background, it was successfully solved by sandwiched a thin layer of the amorphous fluoropolymer between the glass waveguide and the PDMS microwell. Protein secretion from about 900 cells was successfully observed on the platform.

    Original languageEnglish
    Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    PublisherChemical and Biological Microsystems Society
    Pages831-833
    Number of pages3
    ISBN (Print)9780979806476
    Publication statusPublished - 2014
    Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
    Duration: 2014 Oct 262014 Oct 30

    Other

    Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
    CountryUnited States
    CitySan Antonio
    Period14/10/2614/10/30

    Fingerprint

    Waveguides
    Throughput
    Proteins
    Fluorine containing polymers
    Glass
    Fluorescence microscopy
    Optical waveguides

    Keywords

    • Secretion measurement
    • Single-cell
    • TIRFM

    ASJC Scopus subject areas

    • Control and Systems Engineering

    Cite this

    Shirasaki, Y., Suzuki, N., Yamagishi, M., Nakahara, A., Shoji, S., & Ohara, O. (2014). High-throughput single-cell secretion measurement on an optical wavegude chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 831-833). Chemical and Biological Microsystems Society.

    High-throughput single-cell secretion measurement on an optical wavegude chip. / Shirasaki, Y.; Suzuki, N.; Yamagishi, M.; Nakahara, A.; Shoji, Shuichi; Ohara, O.

    18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 831-833.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Shirasaki, Y, Suzuki, N, Yamagishi, M, Nakahara, A, Shoji, S & Ohara, O 2014, High-throughput single-cell secretion measurement on an optical wavegude chip. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 831-833, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.
    Shirasaki Y, Suzuki N, Yamagishi M, Nakahara A, Shoji S, Ohara O. High-throughput single-cell secretion measurement on an optical wavegude chip. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 831-833
    Shirasaki, Y. ; Suzuki, N. ; Yamagishi, M. ; Nakahara, A. ; Shoji, Shuichi ; Ohara, O. / High-throughput single-cell secretion measurement on an optical wavegude chip. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 831-833
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