High-throughput SNP detection using nano-scale engineered biomagnetite

Tadashi Matsunaga, Kohei Maruyama, Haruko Takeyama, Takahiko Katoh

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    A semi-automated system for the large-scale detection of single nucleotide polymorphisms (SNPs) has been developed based on allele-specific oligonucleotide hybridization and thermal dissociation curve analysis using nano-scale engineered biomagnetite (bacterial magnetic particles; BacMPs). For reliable detection in large numbers of samples, several conditions for the capture of target DNA on nano-sized BacMPs and the denaturation of double-stranded DNA were optimized. The most efficient target DNA capture was observed using short PCR amplicons (69 bp). Captured DNAs were denatured using 50 mM NaOH. With these optimizations, large-scale SNP detection was performed on 822 samples of the transforming growth factor (TGF)-β1 gene, which is rich in both GC content and repetitive sequences. High reliability for the semi-automated BacMP-based SNP detection system was confirmed following comparison to traditional sequencing-based methods.

    Original languageEnglish
    Pages (from-to)2315-2321
    Number of pages7
    JournalBiosensors and Bioelectronics
    Volume22
    Issue number9-10
    DOIs
    Publication statusPublished - 2007 Apr 15

    Fingerprint

    Nucleotides
    Polymorphism
    Single Nucleotide Polymorphism
    DNA
    Throughput
    Denaturation
    Oligonucleotides
    Nucleic Acid Repetitive Sequences
    Base Composition
    Transforming Growth Factors
    Hot Temperature
    Genes
    Alleles
    Polymerase Chain Reaction

    Keywords

    • Automated system
    • Bacterial magnetic particles (BacMPs)
    • Hybridization
    • Nano-scale engineered biomagnetite
    • Single nucleotide polymorphism (SNP)
    • Transforming growth factor (TGF)-β1

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Electrochemistry

    Cite this

    High-throughput SNP detection using nano-scale engineered biomagnetite. / Matsunaga, Tadashi; Maruyama, Kohei; Takeyama, Haruko; Katoh, Takahiko.

    In: Biosensors and Bioelectronics, Vol. 22, No. 9-10, 15.04.2007, p. 2315-2321.

    Research output: Contribution to journalArticle

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