High-throughput SNP detection using nano-scale engineered biomagnetite

Tadashi Matsunaga, Kohei Maruyama, Haruko Takeyama, Takahiko Katoh

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

A semi-automated system for the large-scale detection of single nucleotide polymorphisms (SNPs) has been developed based on allele-specific oligonucleotide hybridization and thermal dissociation curve analysis using nano-scale engineered biomagnetite (bacterial magnetic particles; BacMPs). For reliable detection in large numbers of samples, several conditions for the capture of target DNA on nano-sized BacMPs and the denaturation of double-stranded DNA were optimized. The most efficient target DNA capture was observed using short PCR amplicons (69 bp). Captured DNAs were denatured using 50 mM NaOH. With these optimizations, large-scale SNP detection was performed on 822 samples of the transforming growth factor (TGF)-β1 gene, which is rich in both GC content and repetitive sequences. High reliability for the semi-automated BacMP-based SNP detection system was confirmed following comparison to traditional sequencing-based methods.

Original languageEnglish
Pages (from-to)2315-2321
Number of pages7
JournalBiosensors and Bioelectronics
Volume22
Issue number9-10
DOIs
Publication statusPublished - 2007 Apr 15

Keywords

  • Automated system
  • Bacterial magnetic particles (BacMPs)
  • Hybridization
  • Nano-scale engineered biomagnetite
  • Single nucleotide polymorphism (SNP)
  • Transforming growth factor (TGF)-β1

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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