Abstract
A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol 1,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415mM (59.0 g L-1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol-1).
| Original language | English |
|---|---|
| Pages (from-to) | 381-383 |
| Number of pages | 3 |
| Journal | Chemistry Letters |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 Mar 24 |
ASJC Scopus subject areas
- Chemistry(all)
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Kaneko, A., Ishii, Y., & Kirimura, K. (2011). High-yield production of cis,cis-muconic acid from catechol in aqueous solution by biocatalyst. Chemistry Letters, 40(4), 381-383. https://doi.org/10.1246/cl.2011.381