Abstract
A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol 1,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415mM (59.0 g L-1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol-1).
| Original language | English |
|---|---|
| Pages (from-to) | 381-383 |
| Number of pages | 3 |
| Journal | Chemistry Letters |
| Volume | 40 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2011 |
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ASJC Scopus subject areas
- Chemistry(all)
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High-yield production of cis,cis-muconic acid from catechol in aqueous solution by biocatalyst. / Kaneko, Aya; Ishii, Yoshitaka; Kirimura, Kotaro.
In: Chemistry Letters, Vol. 40, No. 4, 2011, p. 381-383.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-yield production of cis,cis-muconic acid from catechol in aqueous solution by biocatalyst
AU - Kaneko, Aya
AU - Ishii, Yoshitaka
AU - Kirimura, Kotaro
PY - 2011
Y1 - 2011
N2 - A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol 1,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415mM (59.0 g L-1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol-1).
AB - A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol 1,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415mM (59.0 g L-1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol-1).
UR - http://www.scopus.com/inward/record.url?scp=79952810842&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952810842&partnerID=8YFLogxK
U2 - 10.1246/cl.2011.381
DO - 10.1246/cl.2011.381
M3 - Article
AN - SCOPUS:79952810842
VL - 40
SP - 381
EP - 383
JO - Chemistry Letters
JF - Chemistry Letters
SN - 0366-7022
IS - 4
ER -