High-yield production of cis,cis-muconic acid from catechol in aqueous solution by biocatalyst

Aya Kaneko, Yoshitaka Ishii, Kotaro Kirimura

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    A fed-batch process was used to produce cis,cis-muconic acid from catechol by recombinant Escherichia coli cells expressing the catA gene, which encodes the Pseudomonas putida mt-2 catechol 1,2-dioxygenase responsible for catalyzing ortho-cleavage of catechol, as biocatalysts. We succeeded in producing 415mM (59.0 g L-1) cis,cis-muconic acid in aqueous solution without generation of by-products in 12 h under the optimal conditions with successive addition of 10mM catechol. The molar conversion yield based on the amount of consumed catechol was the theoretical value of 100% (mol mol-1).

    Original languageEnglish
    Pages (from-to)381-383
    Number of pages3
    JournalChemistry Letters
    Volume40
    Issue number4
    DOIs
    Publication statusPublished - 2011

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    Enzymes
    Catechol 1,2-Dioxygenase
    Escherichia coli
    Byproducts
    Genes
    Cells
    catechol
    muconic acid

    ASJC Scopus subject areas

    • Chemistry(all)

    Cite this

    High-yield production of cis,cis-muconic acid from catechol in aqueous solution by biocatalyst. / Kaneko, Aya; Ishii, Yoshitaka; Kirimura, Kotaro.

    In: Chemistry Letters, Vol. 40, No. 4, 2011, p. 381-383.

    Research output: Contribution to journalArticle

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