Higher-order structure of bovine mitochondrial tRNASerUGA: Chemical modification and computer modeling

Yoh ichi Watanabe, Gota Kawai, Takashi Yokogawa, Nobuhiro Hayashi, Yoshinori Kumazawa, Takuya Ueda, Kazuya Nishikawa, Ichiro Hirao, Kin ichiro Miura, Kimitsuna Watanabe

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

On the basis of enzymatic probing and phylogenetlc comparison, we have previously proposed that mammalian mitochondrial tRNAsSer (anticodon UGA) possess a slightly altered cloverleaf structure In which only one nucleotide exists between the acceptor stem and D stem (usually two nucleotides) and the anticodon stem consists of six base pairs (usually five base pairs) [Yokogawa et al. (1991) Nucleic Acids Res. 19, 6101-6105]. To ascertain whether such tRNAsSer can be folded Into a normal L-shaped tertiary structure, the higher-order structure of bovine mitochondrial tRNASerUGA was examined by chemical probing using dimethylsulfate and dlethylpyrocarbonate, and on the basis of the results a tertiary structure model was obtained by computer modeling. It was found that a one-base-pair elongation In the anticodon stem was compensated for by multiple-base deletions In the D and extra loop regions of the tRNASerUGA, which resulted In preservation of an L-shaped tertiary structure similar to that of conventional tRNAs. By summarizing the findings, the general structural requirements of mitochondrial tRNAs necessary for their functioning in the mitochondrial translation system are considered.

Original languageEnglish
Pages (from-to)5378-5384
Number of pages7
JournalNucleic acids research
Volume22
Issue number24
DOIs
Publication statusPublished - 1994 Dec 1
Externally publishedYes

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Anticodon
Base Pairing
Transfer RNA
Nucleotides
Nucleic Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Watanabe, Y. I., Kawai, G., Yokogawa, T., Hayashi, N., Kumazawa, Y., Ueda, T., ... Watanabe, K. (1994). Higher-order structure of bovine mitochondrial tRNASerUGA: Chemical modification and computer modeling. Nucleic acids research, 22(24), 5378-5384. https://doi.org/10.1093/nar/22.24.5378

Higher-order structure of bovine mitochondrial tRNASerUGA : Chemical modification and computer modeling. / Watanabe, Yoh ichi; Kawai, Gota; Yokogawa, Takashi; Hayashi, Nobuhiro; Kumazawa, Yoshinori; Ueda, Takuya; Nishikawa, Kazuya; Hirao, Ichiro; Miura, Kin ichiro; Watanabe, Kimitsuna.

In: Nucleic acids research, Vol. 22, No. 24, 01.12.1994, p. 5378-5384.

Research output: Contribution to journalArticle

Watanabe, YI, Kawai, G, Yokogawa, T, Hayashi, N, Kumazawa, Y, Ueda, T, Nishikawa, K, Hirao, I, Miura, KI & Watanabe, K 1994, 'Higher-order structure of bovine mitochondrial tRNASerUGA: Chemical modification and computer modeling', Nucleic acids research, vol. 22, no. 24, pp. 5378-5384. https://doi.org/10.1093/nar/22.24.5378
Watanabe, Yoh ichi ; Kawai, Gota ; Yokogawa, Takashi ; Hayashi, Nobuhiro ; Kumazawa, Yoshinori ; Ueda, Takuya ; Nishikawa, Kazuya ; Hirao, Ichiro ; Miura, Kin ichiro ; Watanabe, Kimitsuna. / Higher-order structure of bovine mitochondrial tRNASerUGA : Chemical modification and computer modeling. In: Nucleic acids research. 1994 ; Vol. 22, No. 24. pp. 5378-5384.
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