Highly efficient lipid production in the green alga Parachlorella kessleri: Draft genome and transcriptome endorsed by whole-cell 3D ultrastructure

Shuhei Ota, Kenshiro Oshima, Tomokazu Yamazaki, Sangwan Kim, Zhe Yu, Mai Yoshihara, Kohei Takeda, Tsuyoshi Takeshita, Aiko Hirata, Kateřina Bišová, Vilém Zachleder, Masahira Hattori, Shigeyuki Kawano

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Background: Algae have attracted attention as sustainable producers of lipid-containing biomass for food, animal feed, and for biofuels. Parachlorella kessleri, a unicellular green alga belonging to the class Trebouxiophyceae, achieves very high biomass, lipid, and starch productivity levels. However, further biotechnological exploitation has been hampered by a lack of genomic information. Results: Here, we sequenced the whole genome and transcriptome, and analyzed the behavior of P. kessleri NIES-2152 under lipid production-inducing conditions. The assembly includes 13,057 protein-coding genes in a 62.5-Mbp nuclear genome. Under conditions of sulfur deprivation, lipid accumulation was correlated with the transcriptomic induction of enzymes involved in sulfur metabolism, triacylglycerol (TAG) synthesis, autophagy, and remodeling of light-harvesting complexes. Conclusions: Three-dimensional transmission electron microscopy (3D-TEM) revealed extensive alterations in cellular anatomy accompanying lipid hyperaccumulation. The present 3D-TEM results, together with transcriptomic data support the finding that upregulation of TAG synthesis and autophagy are potential key mediators of the hyperaccumulation of lipids under conditions of nutrient stress.

Original languageEnglish
Article number13
JournalBiotechnology for Biofuels
Volume9
Issue number1
DOIs
Publication statusPublished - 2016 Jan 25
Externally publishedYes

Keywords

  • 3D-TEM
  • Genome
  • Green alga
  • Lipid body
  • Parachlorella kessleri
  • RNA-seq
  • Transcriptome
  • Whole-genome sequence

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Energy(all)
  • Management, Monitoring, Policy and Law

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  • Cite this

    Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., & Kawano, S. (2016). Highly efficient lipid production in the green alga Parachlorella kessleri: Draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnology for Biofuels, 9(1), [13]. https://doi.org/10.1186/s13068-016-0424-2