Highly sensitive detection of allergen using bacterial magnetic particles

N. Nakamura; Tadashi Matsunaga

doi:10.1016/0003-2670(93)85018-F

Highly sensitive detection of allergen using bacterial magnetic particles

N. Nakamura, Tadashi Matsunaga

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml^0-1.

Original language	English
Pages (from-to)	585-589
Number of pages	5
Journal	Analytica Chimica Acta
Volume	281
Issue number	3
DOIs	https://doi.org/10.1016/0003-2670(93)85018-F
Publication status	Published - 1993 Sep 24
Externally published	Yes

Keywords

Allergens
Bacterial magnetic particles
Flourimetry
Immunoassay
Magnetic bacteria

ASJC Scopus subject areas

Biochemistry
Analytical Chemistry
Spectroscopy
Environmental Chemistry

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AU - Matsunaga, Tadashi

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AB - A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.

KW - Allergens

KW - Bacterial magnetic particles

KW - Flourimetry

KW - Immunoassay

KW - Magnetic bacteria

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Highly sensitive detection of allergen using bacterial magnetic particles

Abstract

Keywords

ASJC Scopus subject areas

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