Abstract
A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.
| Original language | English |
|---|---|
| Pages (from-to) | 585-589 |
| Number of pages | 5 |
| Journal | Analytica Chimica Acta |
| Volume | 281 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1993 Sep 24 |
| Externally published | Yes |
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Keywords
- Allergens
- Bacterial magnetic particles
- Flourimetry
- Immunoassay
- Magnetic bacteria
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Highly sensitive detection of allergen using bacterial magnetic particles. / Nakamura, N.; Matsunaga, Tadashi.
In: Analytica Chimica Acta, Vol. 281, No. 3, 24.09.1993, p. 585-589.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Highly sensitive detection of allergen using bacterial magnetic particles
AU - Nakamura, N.
AU - Matsunaga, Tadashi
PY - 1993/9/24
Y1 - 1993/9/24
N2 - A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.
AB - A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.
KW - Allergens
KW - Bacterial magnetic particles
KW - Flourimetry
KW - Immunoassay
KW - Magnetic bacteria
UR - http://www.scopus.com/inward/record.url?scp=0027303208&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027303208&partnerID=8YFLogxK
U2 - 10.1016/0003-2670(93)85018-F
DO - 10.1016/0003-2670(93)85018-F
M3 - Article
AN - SCOPUS:0027303208
VL - 281
SP - 585
EP - 589
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 3
ER -