Abstract
A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.
Original language | English |
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Pages (from-to) | 585-589 |
Number of pages | 5 |
Journal | Analytica Chimica Acta |
Volume | 281 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1993 Sep 24 |
Externally published | Yes |
Keywords
- Allergens
- Bacterial magnetic particles
- Flourimetry
- Immunoassay
- Magnetic bacteria
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry