Abstract
A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.
| Original language | English |
|---|---|
| Pages (from-to) | 585-589 |
| Number of pages | 5 |
| Journal | Analytica Chimica Acta |
| Volume | 281 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1993 Sep 24 |
| Externally published | Yes |
Keywords
- Allergens
- Bacterial magnetic particles
- Flourimetry
- Immunoassay
- Magnetic bacteria
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
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Cite this
Nakamura, N., & Matsunaga, T. (1993). Highly sensitive detection of allergen using bacterial magnetic particles. Analytica Chimica Acta, 281(3), 585-589. https://doi.org/10.1016/0003-2670(93)85018-F