Highly sensitive detection of allergen using bacterial magnetic particles

N. Nakamura, Tadashi Matsunaga

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

A fluoroimmunoassay method using magnetic particles for the highly sensitive detection of allergen was developed. Fluorescein isothiocyanate (FITC)-conjugated IgE antibody was immobilized on bacterial magnetic particles using a heterobifunctional reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The decrease in fluorescence intensity on aggregation of FITC-labelled bacterial magnetic conjugated was determined by spectrofluorimetry. The relative flourescence intensity decreased with increasing allergen concentration. A linear relationship was obtained between the relative flourescence intensity and allergen in the range of 0.5-100 pg ml0-1.

Original languageEnglish
Pages (from-to)585-589
Number of pages5
JournalAnalytica Chimica Acta
Volume281
Issue number3
DOIs
Publication statusPublished - 1993 Sep 24
Externally publishedYes

Keywords

  • Allergens
  • Bacterial magnetic particles
  • Flourimetry
  • Immunoassay
  • Magnetic bacteria

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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