Highly sensitive detection of platelet-derived growth factor on a functionalized diamond surface using aptamer sandwich design

A. Rahim Ruslinda, Varun Penmatsa, Yoko Ishii, Shinya Tajima, Hiroshi Kawarada

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Aptamer-based fluorescence detection of platelet-derived growth factor (PDGF) on a functionalized diamond surface was demonstrated. In this work, a sandwich design based on the ability of PDGF to bind with aptamers at its two available binding sites was employed. It was found that this sandwich design approach significantly increases the fluorescence signal intensity, and thereby a very low detection limit of 4 pM was achieved. The effect of the ionic strength of MgCl2 buffer solution was also investigated, and the most favourable binding for PDGF-BB occurred at a Mg2+ concentration of 5.5 mM. Since the aptamers bind to the target PDGF with high affinity, fluorescence detection exhibited high selectivity towards different biomolecules. The high reproducibility of detection was confirmed by performing three cycles of measurements over a period of three days.

Original languageEnglish
Pages (from-to)1692-1697
Number of pages6
JournalAnalyst
Volume137
Issue number7
DOIs
Publication statusPublished - 2012 Apr 7

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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