Highly sensitive microbiosensor for creatinine based on the combination of inactive polypyrrole with polyion complexes

Tetsuya Osaka; Shinichi Komaba; Akio Amano

doi:10.1149/1.1838277

Highly sensitive microbiosensor for creatinine based on the combination of inactive polypyrrole with polyion complexes

Tetsuya Osaka^*, Shinichi Komaba, Akio Amano

^*Corresponding author for this work

Research Organization for Nano & Life Innovation

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

Potentiometric creatinine microsensors were fabricated by use of a composite film consisting of polyion complex (PIC) which contains creatinine iminohydrolase (CIH), and the electrochemically inactive polypyrrole (iPPy). The PPy/PIC-CIH composite film electrode displayed potential response to creatinine concentration owing to a pH change occurring during the enzymatic reaction. The sensitivity was varied by using different polyions of the PIC. With poly-L-lysine and sebacic acid, the iPPy/PIC-CIH electrode demonstrated a high sensitivity (29.8 mV decade^-1) and a low detection limit (1 × 10^-6 mol dm^-3), which allow one to monitor creatinine concentration in the physiological range.

Original language	English
Pages (from-to)	406-408
Number of pages	3
Journal	Journal of the Electrochemical Society
Volume	145
Issue number	2
DOIs	https://doi.org/10.1149/1.1838277
Publication status	Published - 1998 Feb

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Renewable Energy, Sustainability and the Environment
Surfaces, Coatings and Films
Electrochemistry
Materials Chemistry

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title = "Highly sensitive microbiosensor for creatinine based on the combination of inactive polypyrrole with polyion complexes",

abstract = "Potentiometric creatinine microsensors were fabricated by use of a composite film consisting of polyion complex (PIC) which contains creatinine iminohydrolase (CIH), and the electrochemically inactive polypyrrole (iPPy). The PPy/PIC-CIH composite film electrode displayed potential response to creatinine concentration owing to a pH change occurring during the enzymatic reaction. The sensitivity was varied by using different polyions of the PIC. With poly-L-lysine and sebacic acid, the iPPy/PIC-CIH electrode demonstrated a high sensitivity (29.8 mV decade-1) and a low detection limit (1 × 10-6 mol dm-3), which allow one to monitor creatinine concentration in the physiological range.",

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AU - Osaka, Tetsuya

AU - Komaba, Shinichi

AU - Amano, Akio

PY - 1998/2

Y1 - 1998/2

N2 - Potentiometric creatinine microsensors were fabricated by use of a composite film consisting of polyion complex (PIC) which contains creatinine iminohydrolase (CIH), and the electrochemically inactive polypyrrole (iPPy). The PPy/PIC-CIH composite film electrode displayed potential response to creatinine concentration owing to a pH change occurring during the enzymatic reaction. The sensitivity was varied by using different polyions of the PIC. With poly-L-lysine and sebacic acid, the iPPy/PIC-CIH electrode demonstrated a high sensitivity (29.8 mV decade-1) and a low detection limit (1 × 10-6 mol dm-3), which allow one to monitor creatinine concentration in the physiological range.

AB - Potentiometric creatinine microsensors were fabricated by use of a composite film consisting of polyion complex (PIC) which contains creatinine iminohydrolase (CIH), and the electrochemically inactive polypyrrole (iPPy). The PPy/PIC-CIH composite film electrode displayed potential response to creatinine concentration owing to a pH change occurring during the enzymatic reaction. The sensitivity was varied by using different polyions of the PIC. With poly-L-lysine and sebacic acid, the iPPy/PIC-CIH electrode demonstrated a high sensitivity (29.8 mV decade-1) and a low detection limit (1 × 10-6 mol dm-3), which allow one to monitor creatinine concentration in the physiological range.

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Highly sensitive microbiosensor for creatinine based on the combination of inactive polypyrrole with polyion complexes

Abstract

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