Highly sensitive microbiosensor for creatinine based on the combination of inactive polypyrrole with polyion complexes

Tetsuya Osaka, Shinichi Komaba, Akio Amano

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Potentiometric creatinine microsensors were fabricated by use of a composite film consisting of polyion complex (PIC) which contains creatinine iminohydrolase (CIH), and the electrochemically inactive polypyrrole (iPPy). The PPy/PIC-CIH composite film electrode displayed potential response to creatinine concentration owing to a pH change occurring during the enzymatic reaction. The sensitivity was varied by using different polyions of the PIC. With poly-L-lysine and sebacic acid, the iPPy/PIC-CIH electrode demonstrated a high sensitivity (29.8 mV decade -1) and a low detection limit (1 × 10 -6 mol dm -3), which allow one to monitor creatinine concentration in the physiological range.

Original languageEnglish
Pages (from-to)406-408
Number of pages3
JournalJournal of the Electrochemical Society
Volume145
Issue number2
Publication statusPublished - 1998 Feb

Fingerprint

creatinine deiminase
creatinine
polypyrroles
Polypyrroles
Composite films
Creatinine
Microsensors
Electrodes
Lysine
Acids
sebacic acid
composite materials
electrodes
lysine
sensitivity
polypyrrole

ASJC Scopus subject areas

  • Electrochemistry
  • Surfaces, Coatings and Films
  • Surfaces and Interfaces

Cite this

Highly sensitive microbiosensor for creatinine based on the combination of inactive polypyrrole with polyion complexes. / Osaka, Tetsuya; Komaba, Shinichi; Amano, Akio.

In: Journal of the Electrochemical Society, Vol. 145, No. 2, 02.1998, p. 406-408.

Research output: Contribution to journalArticle

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