Highly thermosensitive Ca 2+ dynamics in a HeLa cell through IP 3 receptors

Vadim Tseeb, Madoka Suzuki, Kotaro Oyama, Kaoru Iwai, Shin'ichi Ishiwata

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Intracellular Ca 2+ distribution and its dynamics are essential for various cellular functions. We show with single HeLa cells that a microscopic heat pulse induces Ca 2+ uptake into intracellular stores during heating and Ca 2+ release from them at the onset of recooling, and the overshoot of Ca 2+ release occurs above the critical value of a temperature change, which decreases from 1.5 to 0.2 °C on increasing the experimental temperature from 22 to 37 °C. This highly thermosensitive Ca 2+ dynamics is probably attributable to the altered balance between Ca 2+ uptake by endoplasmic reticulum Ca 2+-ATPases and Ca 2+ release via inositol 1,4,5-trisphosphate receptors. These results suggest that Ca 2+ signaling is extremely sensitive to temperature changes, especially around body temperature, in cells expressing inositol 1,4,5-trisphosphate receptors.

Original languageEnglish
Pages (from-to)117-123
Number of pages7
JournalHFSP Journal
Volume3
Issue number2
DOIs
Publication statusPublished - 2009 Apr

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HeLa Cells
Inositol 1,4,5-Trisphosphate Receptors
Temperature
Body Temperature
Endoplasmic Reticulum
Heating
Adenosine Triphosphatases
Hot Temperature
Cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)

Cite this

Tseeb, V., Suzuki, M., Oyama, K., Iwai, K., & Ishiwata, S. (2009). Highly thermosensitive Ca 2+ dynamics in a HeLa cell through IP 3 receptors. HFSP Journal, 3(2), 117-123. https://doi.org/10.2976/1.3073779

Highly thermosensitive Ca 2+ dynamics in a HeLa cell through IP 3 receptors. / Tseeb, Vadim; Suzuki, Madoka; Oyama, Kotaro; Iwai, Kaoru; Ishiwata, Shin'ichi.

In: HFSP Journal, Vol. 3, No. 2, 04.2009, p. 117-123.

Research output: Contribution to journalArticle

Tseeb, V, Suzuki, M, Oyama, K, Iwai, K & Ishiwata, S 2009, 'Highly thermosensitive Ca 2+ dynamics in a HeLa cell through IP 3 receptors', HFSP Journal, vol. 3, no. 2, pp. 117-123. https://doi.org/10.2976/1.3073779
Tseeb, Vadim ; Suzuki, Madoka ; Oyama, Kotaro ; Iwai, Kaoru ; Ishiwata, Shin'ichi. / Highly thermosensitive Ca 2+ dynamics in a HeLa cell through IP 3 receptors. In: HFSP Journal. 2009 ; Vol. 3, No. 2. pp. 117-123.
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