HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment

Hiroaki Tachiwana, Sebastian Müller, Julia Blümer, Kerstin Klare, Andrea Musacchio, Geneviève Almouzni

Research output: Contribution to journalArticle

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Abstract

Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3<sup>CENP-A</sup> in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3<sup>CENP-A</sup> contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores. We find that de novo CENP-T accumulation depends on CENP-C and that recruitment of these factors requires two domains in CenH3<sup>CENP-A</sup>: the HJURP-binding region (CATD) and the CENP-C-binding region (CAC). Notably, HJURP interacts directly with CENP-C and is critical for de novo accumulation of CENP-C at synthetic centromeres. On the basis of our findings, we propose that HJURP serves a dual chaperone function in coordinating CenH3<sup>CENP-A</sup> and CENP-C recruitment.

Original languageEnglish
Pages (from-to)22-32
Number of pages11
JournalCell Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2015 Apr 7
Externally publishedYes

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Centromere
Kinetochores
Eukaryota
Histones
centromere protein C
Maintenance

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tachiwana, H., Müller, S., Blümer, J., Klare, K., Musacchio, A., & Almouzni, G. (2015). HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment. Cell Reports, 11(1), 22-32. https://doi.org/10.1016/j.celrep.2015.03.013

HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment. / Tachiwana, Hiroaki; Müller, Sebastian; Blümer, Julia; Klare, Kerstin; Musacchio, Andrea; Almouzni, Geneviève.

In: Cell Reports, Vol. 11, No. 1, 07.04.2015, p. 22-32.

Research output: Contribution to journalArticle

Tachiwana, H, Müller, S, Blümer, J, Klare, K, Musacchio, A & Almouzni, G 2015, 'HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment', Cell Reports, vol. 11, no. 1, pp. 22-32. https://doi.org/10.1016/j.celrep.2015.03.013
Tachiwana H, Müller S, Blümer J, Klare K, Musacchio A, Almouzni G. HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment. Cell Reports. 2015 Apr 7;11(1):22-32. https://doi.org/10.1016/j.celrep.2015.03.013
Tachiwana, Hiroaki ; Müller, Sebastian ; Blümer, Julia ; Klare, Kerstin ; Musacchio, Andrea ; Almouzni, Geneviève. / HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment. In: Cell Reports. 2015 ; Vol. 11, No. 1. pp. 22-32.
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