HJURP involvement in de novo CenH3<sup>CENP-A</sup> and CENP-C recruitment

Hiroaki Tachiwana, Sebastian Müller, Julia Blümer, Kerstin Klare, Andrea Musacchio, Geneviève Almouzni*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

Although our understanding of centromere maintenance, marked by the histone H3 variant CenH3<sup>CENP-A</sup> in most eukaryotes, has progressed, the mechanism underlying the de novo formation of centromeres remains unclear. We used a synthetic system to dissect how CenH3<sup>CENP-A</sup> contributes to the accumulation of CENP-C and CENP-T, two key components that are necessary for the formation of functional kinetochores. We find that de novo CENP-T accumulation depends on CENP-C and that recruitment of these factors requires two domains in CenH3<sup>CENP-A</sup>: the HJURP-binding region (CATD) and the CENP-C-binding region (CAC). Notably, HJURP interacts directly with CENP-C and is critical for de novo accumulation of CENP-C at synthetic centromeres. On the basis of our findings, we propose that HJURP serves a dual chaperone function in coordinating CenH3<sup>CENP-A</sup> and CENP-C recruitment.

Original languageEnglish
Pages (from-to)22-32
Number of pages11
JournalCell Reports
Volume11
Issue number1
DOIs
Publication statusPublished - 2015 Apr 7
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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