HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG

Yusuke Nozaki, Noriko Matsunaga, Toshihiro Ishizawa, Takuya Ueda, Nono Takeuchi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

While all essential mammalian mitochondrial factors involved in the initiation and elongation phases of translation have been cloned and well characterized, little is known about the factors involved in the termination process. In the present work, we report the functional analysis of human mitochondrial translation release factors (RF). Here, we show that HMRF1, which had been previously denoted as a human mitochondrial RF, was inactive in in vitro translation system, although it is a mitochondrial protein. Instead, we identified another human mitochondrial RF candidate, HMRF1L, and demonstrated that HMRF1L is indeed a mitochondrial protein that functions specifically as an RF for the decoding of mitochondrial UAA and UAG termination codons in vitro. The identification of the functional mitochondrial RF brings us much closer to a detailed understanding of the translational termination process in mammalian mitochondria as well as to the unraveling of the molecular mechanism of diseases caused by the dys-regulation of translational termination in human mitochondria.

Original languageEnglish
Pages (from-to)429-438
Number of pages10
JournalGenes to Cells
Volume13
Issue number5
DOIs
Publication statusPublished - 2008 May 1
Externally publishedYes

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Terminator Codon
Mitochondrial Proteins
Mitochondria
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG. / Nozaki, Yusuke; Matsunaga, Noriko; Ishizawa, Toshihiro; Ueda, Takuya; Takeuchi, Nono.

In: Genes to Cells, Vol. 13, No. 5, 01.05.2008, p. 429-438.

Research output: Contribution to journalArticle

Nozaki, Yusuke ; Matsunaga, Noriko ; Ishizawa, Toshihiro ; Ueda, Takuya ; Takeuchi, Nono. / HMRF1L is a human mitochondrial translation release factor involved in the decoding of the termination codons UAA and UAG. In: Genes to Cells. 2008 ; Vol. 13, No. 5. pp. 429-438.
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