Holliday junction binding activity of the human Rad51B protein

Hiroshi Yokoyama, Hitoshi Kurumizaka, Shukuko Ikawa, Shigeyuki Yokoyama, Takehiko Shibata

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The human Rad51B protein is involved in the recombinational repair of damaged DNA. Chromosomal rearrangements of the Rad51B gene have been found in uterine leiomyoma patients, suggesting that the Rad51B gene suppresses tumorigenesis. In the present study, we found that the purified Rad51B protein bound to single-stranded DNA and double-stranded DNA in the presence of ATP and either Mg2+ or Mn2+ and hydrolyzed ATP in a DNA-dependent manner. When the synthetic Holliday junction was present along with the half-cruciform and double-stranded oligonucleotides, the Rad51B protein only bound to the synthetic Holliday junction, which mimics a key intermediate in homologous recombination. In contrast, the human Rad51 protein bound to all three DNA substrates with no obvious preference. Therefore, the Rad51B protein may have a specific function in Holliday junction processing in the homologous recombinational repair pathway in humans.

Original languageEnglish
Pages (from-to)2767-2772
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number4
DOIs
Publication statusPublished - 2003 Jan 24
Externally publishedYes

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Cruciform DNA
Human Activities
Recombinational DNA Repair
DNA
Proteins
Repair
Genes
Adenosine Triphosphate
Gene Rearrangement
Single-Stranded DNA
Homologous Recombination
Leiomyoma
Oligonucleotides
Carcinogenesis
Substrates
Processing

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yokoyama, H., Kurumizaka, H., Ikawa, S., Yokoyama, S., & Shibata, T. (2003). Holliday junction binding activity of the human Rad51B protein. Journal of Biological Chemistry, 278(4), 2767-2772. https://doi.org/10.1074/jbc.M210899200

Holliday junction binding activity of the human Rad51B protein. / Yokoyama, Hiroshi; Kurumizaka, Hitoshi; Ikawa, Shukuko; Yokoyama, Shigeyuki; Shibata, Takehiko.

In: Journal of Biological Chemistry, Vol. 278, No. 4, 24.01.2003, p. 2767-2772.

Research output: Contribution to journalArticle

Yokoyama, H, Kurumizaka, H, Ikawa, S, Yokoyama, S & Shibata, T 2003, 'Holliday junction binding activity of the human Rad51B protein', Journal of Biological Chemistry, vol. 278, no. 4, pp. 2767-2772. https://doi.org/10.1074/jbc.M210899200
Yokoyama H, Kurumizaka H, Ikawa S, Yokoyama S, Shibata T. Holliday junction binding activity of the human Rad51B protein. Journal of Biological Chemistry. 2003 Jan 24;278(4):2767-2772. https://doi.org/10.1074/jbc.M210899200
Yokoyama, Hiroshi ; Kurumizaka, Hitoshi ; Ikawa, Shukuko ; Yokoyama, Shigeyuki ; Shibata, Takehiko. / Holliday junction binding activity of the human Rad51B protein. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 4. pp. 2767-2772.
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