Hollow-fiber blood-dialysis membranes: Superoxide generation, permeation, and dismutation measured by chemiluminescence

Ken Ichiro Yamamoto, Kazuyoshi Kobayashi, Kosuke Endo, Takehiro Miyasaka, Seiichi Mochizuki, Fukashi Kohori, Kiyotaka Sakai

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    The interaction of blood with a material surface results in activation of the body's humoral immune system and the generation of reactive oxygen species (ROS). It has recently become clear that ROS are central to the pathology of many diseases. In this study, we evaluated the superoxide generation, permeation, and dismutation in hollow-fiber dialysis membranes by using 2-methyl-6-p-methoxyphenylethynyl-imidazopyrazinone (MPEC) as a superoxide-reactive chemiluminescence producer and an optical fiber probe to detect the resulting chemiluminescence in the hollow fiber lumen. We measured the superoxide generated when bovine blood leukocytes were brought into contact with dialysis membranes. Superoxide permeation was determined by measuring MPEC chemiluminescence in the hollow fiber lumen using an optical fiber probe. Additionally, superoxide dismutation was evaluated by examining the difference in superoxide permeability for membranes with and without vitamin E coating. Superoxide generation varies for different membrane materials, depending on the membrane's biocompatibility. Superoxide permeability depends on the diffusive permeability of membranes. No marked decrease in superoxide permeability was observed among membrane materials. The superoxide permeability of vitamin E-coated membrane was smaller than that of uncoated membrane. The antioxidant property of vitamin E-coated membranes is hence effective in causing superoxide dismutation.

    Original languageEnglish
    Pages (from-to)257-262
    Number of pages6
    JournalJournal of Artificial Organs
    Volume8
    Issue number4
    DOIs
    Publication statusPublished - 2005 Dec

    Fingerprint

    Dialysis membranes
    Chemiluminescence
    Luminescence
    Permeation
    Superoxides
    Dialysis
    Blood
    Membranes
    Fibers
    Vitamins
    Permeability
    Vitamin E
    Optical Fibers
    Optical fibers
    Reactive Oxygen Species
    Oxygen
    Immune system
    Pathology
    Antioxidants
    Biocompatibility

    Keywords

    • 2-Methyl-6-p- methoxyphenylethynyl-imidazopyrazinone
    • Chemiluminescence
    • Dialysis membrane
    • Hollow fiber
    • Superoxide

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Hollow-fiber blood-dialysis membranes : Superoxide generation, permeation, and dismutation measured by chemiluminescence. / Yamamoto, Ken Ichiro; Kobayashi, Kazuyoshi; Endo, Kosuke; Miyasaka, Takehiro; Mochizuki, Seiichi; Kohori, Fukashi; Sakai, Kiyotaka.

    In: Journal of Artificial Organs, Vol. 8, No. 4, 12.2005, p. 257-262.

    Research output: Contribution to journalArticle

    Yamamoto, Ken Ichiro ; Kobayashi, Kazuyoshi ; Endo, Kosuke ; Miyasaka, Takehiro ; Mochizuki, Seiichi ; Kohori, Fukashi ; Sakai, Kiyotaka. / Hollow-fiber blood-dialysis membranes : Superoxide generation, permeation, and dismutation measured by chemiluminescence. In: Journal of Artificial Organs. 2005 ; Vol. 8, No. 4. pp. 257-262.
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