Hollow-fiber membrane chamber as a device for in situ environmental cultivation

Yoshiteru Aoi, Tomoyuki Kinoshita, Toru Hata, Hiroaki Ohta, Haruko Obokata, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    53 Citations (Scopus)

    Abstract

    A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

    Original languageEnglish
    Pages (from-to)3826-3833
    Number of pages8
    JournalApplied and Environmental Microbiology
    Volume75
    Issue number11
    DOIs
    Publication statusPublished - 2009 Jun

    Fingerprint

    agar
    membrane
    microorganisms
    injectors
    Equipment and Supplies
    Membranes
    chemical compounds
    Agar
    sampling
    microorganism
    chemical compound
    in situ
    fibre
    phylotype

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology
    • Food Science
    • Biotechnology
    • Ecology

    Cite this

    Hollow-fiber membrane chamber as a device for in situ environmental cultivation. / Aoi, Yoshiteru; Kinoshita, Tomoyuki; Hata, Toru; Ohta, Hiroaki; Obokata, Haruko; Tsuneda, Satoshi.

    In: Applied and Environmental Microbiology, Vol. 75, No. 11, 06.2009, p. 3826-3833.

    Research output: Contribution to journalArticle

    Aoi, Yoshiteru ; Kinoshita, Tomoyuki ; Hata, Toru ; Ohta, Hiroaki ; Obokata, Haruko ; Tsuneda, Satoshi. / Hollow-fiber membrane chamber as a device for in situ environmental cultivation. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 11. pp. 3826-3833.
    @article{3f62fb401c424d1098e16ee9564e2344,
    title = "Hollow-fiber membrane chamber as a device for in situ environmental cultivation",
    abstract = "A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.",
    author = "Yoshiteru Aoi and Tomoyuki Kinoshita and Toru Hata and Hiroaki Ohta and Haruko Obokata and Satoshi Tsuneda",
    year = "2009",
    month = "6",
    doi = "10.1128/AEM.02542-08",
    language = "English",
    volume = "75",
    pages = "3826--3833",
    journal = "Applied and Environmental Microbiology",
    issn = "0099-2240",
    publisher = "American Society for Microbiology",
    number = "11",

    }

    TY - JOUR

    T1 - Hollow-fiber membrane chamber as a device for in situ environmental cultivation

    AU - Aoi, Yoshiteru

    AU - Kinoshita, Tomoyuki

    AU - Hata, Toru

    AU - Ohta, Hiroaki

    AU - Obokata, Haruko

    AU - Tsuneda, Satoshi

    PY - 2009/6

    Y1 - 2009/6

    N2 - A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

    AB - A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

    UR - http://www.scopus.com/inward/record.url?scp=66249144102&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=66249144102&partnerID=8YFLogxK

    U2 - 10.1128/AEM.02542-08

    DO - 10.1128/AEM.02542-08

    M3 - Article

    VL - 75

    SP - 3826

    EP - 3833

    JO - Applied and Environmental Microbiology

    JF - Applied and Environmental Microbiology

    SN - 0099-2240

    IS - 11

    ER -