Hollow-fiber membrane chamber as a device for in situ environmental cultivation

Yoshiteru Aoi*, Tomoyuki Kinoshita, Toru Hata, Hiroaki Ohta, Haruko Obokata, Satoshi Tsuneda

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)


A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

Original languageEnglish
Pages (from-to)3826-3833
Number of pages8
JournalApplied and Environmental Microbiology
Issue number11
Publication statusPublished - 2009 Jun

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology


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