Hollow-fiber membrane chamber as a device for in situ environmental cultivation

Yoshiteru Aoi; Tomoyuki Kinoshita; Toru Hata; Hiroaki Ohta; Haruko Obokata; Satoshi Tsuneda

doi:10.1128/AEM.02542-08

Hollow-fiber membrane chamber as a device for in situ environmental cultivation

Yoshiteru Aoi, Tomoyuki Kinoshita, Toru Hata, Hiroaki Ohta, Haruko Obokata, Satoshi Tsuneda

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54 Citations (Scopus)

Abstract

A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

Original language	English
Pages (from-to)	3826-3833
Number of pages	8
Journal	Applied and Environmental Microbiology
Volume	75
Issue number	11
DOIs	https://doi.org/10.1128/AEM.02542-08
Publication status	Published - 2009 Jun

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ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Food Science
Biotechnology
Ecology

Cite this

Aoi, Y., Kinoshita, T., Hata, T., Ohta, H., Obokata, H., & Tsuneda, S. (2009). Hollow-fiber membrane chamber as a device for in situ environmental cultivation. Applied and Environmental Microbiology, 75(11), 3826-3833. https://doi.org/10.1128/AEM.02542-08

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AU - Tsuneda, Satoshi

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10.1128/AEM.02542-08