Hollow-fiber membrane chamber as a device for in situ environmental cultivation

Yoshiteru Aoi, Tomoyuki Kinoshita, Toru Hata, Hiroaki Ohta, Haruko Obokata, Satoshi Tsuneda

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    Abstract

    A hollow-fiber membrane chamber (HFMC) was developed as an in situ cultivation device for environmental microorganisms. The HFMC system consists of 48 to 96 pieces of porous hollow-fiber membrane connected with injectors. The system allows rapid exchange of chemical compounds, thereby simulating a natural environment. Comparative analysis through the cultivation of three types of environmental samples was performed using this newly designed device and a conventional agar-based petri dish. The results show that the ratios of novel phylotypes in isolates, species-level diversities, and cultivabilities in HFMC-based cultivation are higher than those in an agar-based petri dish for all three samples, suggesting that the new in situ cultivation device is effective for cultivation of various environmental microorganisms.

    Original languageEnglish
    Pages (from-to)3826-3833
    Number of pages8
    JournalApplied and Environmental Microbiology
    Volume75
    Issue number11
    DOIs
    Publication statusPublished - 2009 Jun

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    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology
    • Food Science
    • Biotechnology
    • Ecology

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