Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1

Wataru Kobayashi, Enwei Liu, Hajime Ishii, Sachihiro Matsunaga, Peter Schlögelhofer, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    Abstract

    In eukaryotes, homologous recombination plays a pivotal role in both genome maintenance and generation of genetic diversity. Eukaryotic RecA homologues, RAD51 and DMC1, are key proteins in homologous recombination that promote pairing between homologous DNA sequences. Arabidopsis thaliana is a prominent model plant for studying eukaryotic homologous recombination. However, A. thaliana RAD51 and DMC1 have not been biochemically characterized. In the present study, we purified A. thaliana RAD51 (AtRAD51) and DMC1 (AtDMC1). Biochemical analyses revealed that both AtRAD51 and AtDMC1 possess ATP hydrolyzing activity, filament formation activity and homologous pairing activity in vitro. We then compared the homologous pairing activities of AtRAD51 and AtDMC1 with those of the Oryza sativa and Homo sapiens RAD51 and DMC1 proteins.

    Original languageEnglish
    Pages (from-to)289-295
    Number of pages7
    JournalJournal of biochemistry
    Volume165
    Issue number3
    DOIs
    Publication statusPublished - 2019 Mar 1

    Fingerprint

    Arabidopsis
    Homologous Recombination
    DNA sequences
    Proteins
    Genes
    Adenosine Triphosphate
    Rad51 Recombinase
    Sequence Homology
    Eukaryota
    Maintenance
    Genome

    Keywords

    • Arabidopsis thaliana
    • DMC1
    • homologous pairing
    • homologous recombination
    • RAD51

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

    Cite this

    Kobayashi, W., Liu, E., Ishii, H., Matsunaga, S., Schlögelhofer, P., & Kurumizaka, H. (2019). Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1. Journal of biochemistry, 165(3), 289-295. https://doi.org/10.1093/jb/mvy105

    Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1. / Kobayashi, Wataru; Liu, Enwei; Ishii, Hajime; Matsunaga, Sachihiro; Schlögelhofer, Peter; Kurumizaka, Hitoshi.

    In: Journal of biochemistry, Vol. 165, No. 3, 01.03.2019, p. 289-295.

    Research output: Contribution to journalArticle

    Kobayashi, W, Liu, E, Ishii, H, Matsunaga, S, Schlögelhofer, P & Kurumizaka, H 2019, 'Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1' Journal of biochemistry, vol. 165, no. 3, pp. 289-295. https://doi.org/10.1093/jb/mvy105
    Kobayashi W, Liu E, Ishii H, Matsunaga S, Schlögelhofer P, Kurumizaka H. Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1. Journal of biochemistry. 2019 Mar 1;165(3):289-295. https://doi.org/10.1093/jb/mvy105
    Kobayashi, Wataru ; Liu, Enwei ; Ishii, Hajime ; Matsunaga, Sachihiro ; Schlögelhofer, Peter ; Kurumizaka, Hitoshi. / Homologous pairing activities of Arabidopsis thaliana RAD51 and DMC1. In: Journal of biochemistry. 2019 ; Vol. 165, No. 3. pp. 289-295.
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