Homologous Pairing Activities of Two Rice RAD51 Proteins, RAD51A1 and RAD51A2

Yuichi Morozumi, Ryohei Ino, Shukuko Ikawa, Naozumi Mimida, Takeshi Shimizu, Seiichi Toki, Hiroaki Ichikawa, Takehiko Shibata, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    In higher eukaryotes, RAD51 functions as an essential protein in homologous recombination and recombinational repair of DNA double strand breaks. During these processes, RAD51 catalyzes homologous pairing between single-stranded DNA and double-stranded DNA. Japonica cultivars of rice (Oryza sativa) encode two RAD51 proteins, RAD51A1 and RAD51A2, whereas only one RAD51 exists in yeast and mammals. However, the functional differences between RAD51A1 and RAD51A2 have not been elucidated, because their biochemical properties have not been characterized. In the present study, we purified RAD51A1 and RAD51A2, and found that RAD51A2 robustly promotes homologous pairing in vitro. RAD51A1 also possesses homologous-pairing activity, but it is only about 10% of the RAD51A2 activity. Both RAD51A1 and RAD51A2 bind to ssDNA and dsDNA, and their DNA binding strictly requires ATP, which modulates the polymer formation activities of RAD51A1 and RAD51A2. These findings suggest that although both RAD51A1 and RAD51A2 have the potential to catalyze homologous pairing, RAD51A2 may be the major recombinase in rice.

    Original languageEnglish
    Article numbere75451
    JournalPLoS One
    Volume8
    Issue number10
    DOIs
    Publication statusPublished - 2013 Oct 4

    Fingerprint

    Rad51 Recombinase
    Recombinational DNA Repair
    rice protein
    single-stranded DNA
    rice
    homologous recombination
    DNA
    DNA repair
    eukaryotic cells
    Oryza sativa
    polymers
    Recombinases
    Mammals
    proteins
    Single-Stranded DNA
    mammals
    yeasts
    Eukaryota
    Yeast
    Polymers

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Morozumi, Y., Ino, R., Ikawa, S., Mimida, N., Shimizu, T., Toki, S., ... Kurumizaka, H. (2013). Homologous Pairing Activities of Two Rice RAD51 Proteins, RAD51A1 and RAD51A2. PLoS One, 8(10), [e75451]. https://doi.org/10.1371/journal.pone.0075451

    Homologous Pairing Activities of Two Rice RAD51 Proteins, RAD51A1 and RAD51A2. / Morozumi, Yuichi; Ino, Ryohei; Ikawa, Shukuko; Mimida, Naozumi; Shimizu, Takeshi; Toki, Seiichi; Ichikawa, Hiroaki; Shibata, Takehiko; Kurumizaka, Hitoshi.

    In: PLoS One, Vol. 8, No. 10, e75451, 04.10.2013.

    Research output: Contribution to journalArticle

    Morozumi, Y, Ino, R, Ikawa, S, Mimida, N, Shimizu, T, Toki, S, Ichikawa, H, Shibata, T & Kurumizaka, H 2013, 'Homologous Pairing Activities of Two Rice RAD51 Proteins, RAD51A1 and RAD51A2', PLoS One, vol. 8, no. 10, e75451. https://doi.org/10.1371/journal.pone.0075451
    Morozumi Y, Ino R, Ikawa S, Mimida N, Shimizu T, Toki S et al. Homologous Pairing Activities of Two Rice RAD51 Proteins, RAD51A1 and RAD51A2. PLoS One. 2013 Oct 4;8(10). e75451. https://doi.org/10.1371/journal.pone.0075451
    Morozumi, Yuichi ; Ino, Ryohei ; Ikawa, Shukuko ; Mimida, Naozumi ; Shimizu, Takeshi ; Toki, Seiichi ; Ichikawa, Hiroaki ; Shibata, Takehiko ; Kurumizaka, Hitoshi. / Homologous Pairing Activities of Two Rice RAD51 Proteins, RAD51A1 and RAD51A2. In: PLoS One. 2013 ; Vol. 8, No. 10.
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