Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex

Hitoshi Kurumizaka, Shukuko Ikawa, Maki Nakada, Rima Enomoto, Wataru Kagawa, Takashi Kinebuchi, Mitsuyoshi Yamazoe, Shigeyuki Yokoyama, Takehiko Shibata

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The Xrcc2 and Rad51D/Rad51L3 proteins, which belong to the Rad51 paralogs, are required for homologous recombinational repair (HRR) in vertebrates. The Xrcc2 and Rad51D/Rad51L3 genes, whose products interact with each other, have essential roles in ensuring normal embryonic development. In the present study, we coexpressed the human Xrcc2 and Rad51D/Rad51L3 proteins (Xrcc2 and Rad51D, respectively) in Escherichia coli, and purified the Xrcc2·Rad51D complex to homogeneity. The Xrcc2·Rad51D complex catalyzed homologous pairing between single-stranded and double-stranded DNA, similar to the function of the Xrcc3· Rad51C complex, which is another complex of the Rad51 paralogs. An electron microscopic analysis showed that Xrcc2·Rad51D formed a multimeric ring structure in the absence of DNA. In the presence of ssDNA, Xrcc2·Rad-51D formed a filamentous structure, which is commonly observed among the human homologous pairing proteins, Rad51, Rad52, and Xrcc3·Rad51C.

Original languageEnglish
Pages (from-to)14315-14320
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number16
DOIs
Publication statusPublished - 2002 Apr 19
Externally publishedYes

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Human Activities
Rad52 DNA Repair and Recombination Protein
Recombinational DNA Repair
DNA
Embryonic Development
Vertebrates
Proteins
Electrons
Escherichia coli
Repair
Genes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kurumizaka, H., Ikawa, S., Nakada, M., Enomoto, R., Kagawa, W., Kinebuchi, T., ... Shibata, T. (2002). Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex. Journal of Biological Chemistry, 277(16), 14315-14320. https://doi.org/10.1074/jbc.M105719200

Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex. / Kurumizaka, Hitoshi; Ikawa, Shukuko; Nakada, Maki; Enomoto, Rima; Kagawa, Wataru; Kinebuchi, Takashi; Yamazoe, Mitsuyoshi; Yokoyama, Shigeyuki; Shibata, Takehiko.

In: Journal of Biological Chemistry, Vol. 277, No. 16, 19.04.2002, p. 14315-14320.

Research output: Contribution to journalArticle

Kurumizaka, H, Ikawa, S, Nakada, M, Enomoto, R, Kagawa, W, Kinebuchi, T, Yamazoe, M, Yokoyama, S & Shibata, T 2002, 'Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex', Journal of Biological Chemistry, vol. 277, no. 16, pp. 14315-14320. https://doi.org/10.1074/jbc.M105719200
Kurumizaka, Hitoshi ; Ikawa, Shukuko ; Nakada, Maki ; Enomoto, Rima ; Kagawa, Wataru ; Kinebuchi, Takashi ; Yamazoe, Mitsuyoshi ; Yokoyama, Shigeyuki ; Shibata, Takehiko. / Homologous pairing and ring and filament structure formation activities of the human Xrcc2·Rad51D complex. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 16. pp. 14315-14320.
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