Homologous Pairing Promoted by the Human, Rad52 Protein

Wataru Kagawa, Hitoshi Kurumizaka, Shukuko Ikawa, Shigeyuki Yokoyama, Takehiko Shibata

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

The Rad52 protein, which is unique to eukaryotes, plays important roles in the Rad51-dependent and the Rad51-independent pathways of DNA recombination. In the present study, we have biochemically characterized the homologous pairing activity of the HsRad52 protein (Homo sapiens Rad52) and found that the presynaptic complex formation with ssDNA is essential in its catalysis of homologous pairing. We have identified an N-terminal fragment (amino acid residues 1-237, HsRad521-237) that is defective in binding to the human Rad51 protein, which catalyzed homologous pairing as efficiently as the wild type HsRad52. Electron microscopic visualization revealed that HsRad52 and HsRad521-237 both formed nucleoprotein filaments with single-stranded DNA. These lines of evidence suggest the role of HsRad52 in the homologous pairing step of the Rad51-independent recombination pathway. Our results reveal the striking similarity between HsRad52 and the Escherichia coli RecT protein, which functions in a RecA-independent recombination pathway.

Original languageEnglish
Pages (from-to)35201-35208
Number of pages8
JournalJournal of Biological Chemistry
Volume276
Issue number37
DOIs
Publication statusPublished - 2001 Sep 14
Externally publishedYes

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Rad52 DNA Repair and Recombination Protein
Genetic Recombination
Proteins
Nucleoproteins
Escherichia coli Proteins
Single-Stranded DNA
Eukaryota
Catalysis
Escherichia coli
Visualization
Electrons
Amino Acids
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Kagawa, W., Kurumizaka, H., Ikawa, S., Yokoyama, S., & Shibata, T. (2001). Homologous Pairing Promoted by the Human, Rad52 Protein. Journal of Biological Chemistry, 276(37), 35201-35208. https://doi.org/10.1074/jbc.M104938200

Homologous Pairing Promoted by the Human, Rad52 Protein. / Kagawa, Wataru; Kurumizaka, Hitoshi; Ikawa, Shukuko; Yokoyama, Shigeyuki; Shibata, Takehiko.

In: Journal of Biological Chemistry, Vol. 276, No. 37, 14.09.2001, p. 35201-35208.

Research output: Contribution to journalArticle

Kagawa, W, Kurumizaka, H, Ikawa, S, Yokoyama, S & Shibata, T 2001, 'Homologous Pairing Promoted by the Human, Rad52 Protein', Journal of Biological Chemistry, vol. 276, no. 37, pp. 35201-35208. https://doi.org/10.1074/jbc.M104938200
Kagawa W, Kurumizaka H, Ikawa S, Yokoyama S, Shibata T. Homologous Pairing Promoted by the Human, Rad52 Protein. Journal of Biological Chemistry. 2001 Sep 14;276(37):35201-35208. https://doi.org/10.1074/jbc.M104938200
Kagawa, Wataru ; Kurumizaka, Hitoshi ; Ikawa, Shukuko ; Yokoyama, Shigeyuki ; Shibata, Takehiko. / Homologous Pairing Promoted by the Human, Rad52 Protein. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 37. pp. 35201-35208.
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