Homologous radioimmunoassay for plasma and pituitary prolactin in the toad, Bufo japonicus

Kazutoshi Yamamoto, Sakae Kikuyama, Susumu Ishii

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    A specific and sensitive homologous radioimmunoassay (RIA) was developed for the measurement of toad (Bufo japonicus) prolactin (PRL). PRL isolated from toad pituitary glands was used for generating antiserum in a rabbit, for radioiodination, and for the standard. Several dilutions of plasma and a homogenate of the anterior lobe of the pituitary gland of toads yielded dose-response curves which paralleled the standard. Plasma from hypophysectomized toads showed the least amount of cross-reaction. Bovine PRL, ovine PRL, ovine growth hormone, and a homogenate of toad neurointermediate lobes showed no inhibition of binding even at relatively high doses in this RIA. A preliminary application of homologous RIA for toad PRL was performed by determining plasma and pituitary PRL levels in adult toads of both sexes captured in spring and late summer. Plasma PRL levels of male toads remaining in the pond for breeding were significantly higher than those of the males feeding in the bush or hibernating under the ground. The pituitary PRL content in spring toads of both sexes was higher than those in summer toads. The pituitary PRL content in females was invariably lower than those in simultaneously captured males.

    Original languageEnglish
    Pages (from-to)373-376
    Number of pages4
    JournalGeneral and Comparative Endocrinology
    Volume74
    Issue number3
    DOIs
    Publication statusPublished - 1989

    Fingerprint

    Bufonidae
    Bufo
    toads
    prolactin
    radioimmunoassays
    Anura
    Prolactin
    Radioimmunoassay
    Anterior Pituitary Gland
    pituitary gland
    Sheep
    sheep
    gender
    summer
    Cross Reactions
    Pituitary Gland
    cross reaction
    somatotropin
    Growth Hormone
    dose response

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Homologous radioimmunoassay for plasma and pituitary prolactin in the toad, Bufo japonicus. / Yamamoto, Kazutoshi; Kikuyama, Sakae; Ishii, Susumu.

    In: General and Comparative Endocrinology, Vol. 74, No. 3, 1989, p. 373-376.

    Research output: Contribution to journalArticle

    Yamamoto, Kazutoshi ; Kikuyama, Sakae ; Ishii, Susumu. / Homologous radioimmunoassay for plasma and pituitary prolactin in the toad, Bufo japonicus. In: General and Comparative Endocrinology. 1989 ; Vol. 74, No. 3. pp. 373-376.
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