Horizontal gene acquisition of Liberibacter plant pathogens from a bacteriome-confined endosymbiont of their psyllid vector

Atsushi Nakabachi, Naruo Nikoh, Kenshiro Oshima, Hiromitsu Inoue, Moriya Ohkuma, Yuichi Hongoh, Shin Ya Miyagishima, Masahira Hattori, Takema Fukatsu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

He Asian citrus psyllid Diaphorina citri is a notorious agricultural pest that transmits the phloem-inhabiting alphaproteobacterial 'Candidatus Liberibacter asiaticus' and allied plant pathogens, which cause the devastating citrus disease called Huanglongbing or greening disease. D. citri harbors two distinct bacterial mutualists in the symbiotic organ called bacteriome: the betaproteobacterium 'Candidatus Profftella armatura' in the syncytial cytoplasm at the center of the bacteriome, and the gammaproteobacterium 'Candidatus Carsonella ruddii' in uninucleate bacteriocytes. Here we report that a putative amino acid transporter LysE of Profftella forms a highly supported clade with proteins of L. asiaticus, L. americanus, and L. solanacearum. L. crescens, the most basal Liberibacter lineage currently known, lacked the corresponding gene. The Profftella-Liberibacter subclade of LysE formed a clade with proteins from betaproteobacteria of the order Burkholderiales, to which Profftella belongs. This phylogenetic pattern favors the hypothesis that the Liberibacter lineage acquired the gene from the Profftella lineage via horizontal gene transfer (HGT) after L. crescens diverged from other Liberibacter lineages. KA/KS analyses further supported the hypothesis that the genes encoded in the Liberibacter genomes are functional. These findings highlight the possible evolutionary importance of HGT between plant pathogens and their insect vector's symbionts that are confined in the symbiotic organ and seemingly sequestered from external microbial populations.

Original languageEnglish
Article numbere82612
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - 2013 Dec 4
Externally publishedYes

Fingerprint

Candidatus Liberibacter
Hemiptera
Psyllidae
endosymbionts
Pathogens
plant pathogens
Betaproteobacteria
Horizontal Gene Transfer
Diaphorina citri
Citrus
Genes
Gene transfer
Candidatus Liberibacter asiaticus
greening disease
beta-Proteobacteria
Insect Vectors
Gammaproteobacteria
Amino Acid Transport Systems
Phloem
genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nakabachi, A., Nikoh, N., Oshima, K., Inoue, H., Ohkuma, M., Hongoh, Y., ... Fukatsu, T. (2013). Horizontal gene acquisition of Liberibacter plant pathogens from a bacteriome-confined endosymbiont of their psyllid vector. PLoS One, 8(12), [e82612]. https://doi.org/10.1371/journal.pone.0082612

Horizontal gene acquisition of Liberibacter plant pathogens from a bacteriome-confined endosymbiont of their psyllid vector. / Nakabachi, Atsushi; Nikoh, Naruo; Oshima, Kenshiro; Inoue, Hiromitsu; Ohkuma, Moriya; Hongoh, Yuichi; Miyagishima, Shin Ya; Hattori, Masahira; Fukatsu, Takema.

In: PLoS One, Vol. 8, No. 12, e82612, 04.12.2013.

Research output: Contribution to journalArticle

Nakabachi, A, Nikoh, N, Oshima, K, Inoue, H, Ohkuma, M, Hongoh, Y, Miyagishima, SY, Hattori, M & Fukatsu, T 2013, 'Horizontal gene acquisition of Liberibacter plant pathogens from a bacteriome-confined endosymbiont of their psyllid vector', PLoS One, vol. 8, no. 12, e82612. https://doi.org/10.1371/journal.pone.0082612
Nakabachi, Atsushi ; Nikoh, Naruo ; Oshima, Kenshiro ; Inoue, Hiromitsu ; Ohkuma, Moriya ; Hongoh, Yuichi ; Miyagishima, Shin Ya ; Hattori, Masahira ; Fukatsu, Takema. / Horizontal gene acquisition of Liberibacter plant pathogens from a bacteriome-confined endosymbiont of their psyllid vector. In: PLoS One. 2013 ; Vol. 8, No. 12.
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