Hormonal action of relaxin-like gonad-stimulating substance (GSS) on starfish ovaries in growing and fully grown states

Masatoshi Mita, Kazutoshi Yamamoto, Masaru Nakamura, Yoshitaka Nagahama

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Recently, we purified GSS from the radial nerves of the starfish Asterina pectinifera and identified the chemical structure as a relaxin-like peptide. This study examined the hormonal action of GSS on ovaries in the growing (stage IV) and fully grown states (stage V) of the starfish. The sensitivity of oocytes to 1-methyladenine (1-MeAde) as starfish maturation-inducing hormone was enhanced as oocytes enlarged in stage V. GSS-stimulated 1-MeAde production by ovarian follicle cells was also correlated with the size of oocytes. Although 1-MeAde production was observed in whole ovaries in stage V, GSS failed to induce 1-MeAde production in young ovaries (stage IV). This suggests that follicle cells in ovaries in a growing state (stage IV) are still unresponsive to the hormonal action of GSS. According to competitive experiments using radioiodinated and radioinert GSS, however, dissociation constant (Kd) values and the number of binding sites for GSS were mostly constant in the ovaries from stages IV to V. These results strongly suggest that GSS receptors are expressed in follicle cells of ovaries in the growing state. The failure of GSS to induce 1-MeAde production in young ovaries may be due to the uncoupling of signal transduction from the receptor to 1-MeAde biosynthesis in follicle cells.

    Original languageEnglish
    Pages (from-to)85-89
    Number of pages5
    JournalGeneral and Comparative Endocrinology
    Volume172
    Issue number1
    DOIs
    Publication statusPublished - 2011 May 15

    Fingerprint

    relaxin
    Starfish
    Relaxin
    Asteroidea
    Gonads
    gonads
    Ovary
    Oocytes
    oocytes
    Invertebrate Hormones
    Asterina
    Asterina pectinifera
    Radial Nerve
    cells
    Ovarian Follicle
    Peptide Hormones
    peptide hormones
    receptors
    Gonadotropins
    ovarian follicles

    Keywords

    • 1-Methyladenine (1-MeAde)
    • Gonad-stimulating substance (GSS)
    • Gonadotropin
    • Oocyte maturation
    • Starfish

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Hormonal action of relaxin-like gonad-stimulating substance (GSS) on starfish ovaries in growing and fully grown states. / Mita, Masatoshi; Yamamoto, Kazutoshi; Nakamura, Masaru; Nagahama, Yoshitaka.

    In: General and Comparative Endocrinology, Vol. 172, No. 1, 15.05.2011, p. 85-89.

    Research output: Contribution to journalArticle

    Mita, Masatoshi ; Yamamoto, Kazutoshi ; Nakamura, Masaru ; Nagahama, Yoshitaka. / Hormonal action of relaxin-like gonad-stimulating substance (GSS) on starfish ovaries in growing and fully grown states. In: General and Comparative Endocrinology. 2011 ; Vol. 172, No. 1. pp. 85-89.
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    abstract = "Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Recently, we purified GSS from the radial nerves of the starfish Asterina pectinifera and identified the chemical structure as a relaxin-like peptide. This study examined the hormonal action of GSS on ovaries in the growing (stage IV) and fully grown states (stage V) of the starfish. The sensitivity of oocytes to 1-methyladenine (1-MeAde) as starfish maturation-inducing hormone was enhanced as oocytes enlarged in stage V. GSS-stimulated 1-MeAde production by ovarian follicle cells was also correlated with the size of oocytes. Although 1-MeAde production was observed in whole ovaries in stage V, GSS failed to induce 1-MeAde production in young ovaries (stage IV). This suggests that follicle cells in ovaries in a growing state (stage IV) are still unresponsive to the hormonal action of GSS. According to competitive experiments using radioiodinated and radioinert GSS, however, dissociation constant (Kd) values and the number of binding sites for GSS were mostly constant in the ovaries from stages IV to V. These results strongly suggest that GSS receptors are expressed in follicle cells of ovaries in the growing state. The failure of GSS to induce 1-MeAde production in young ovaries may be due to the uncoupling of signal transduction from the receptor to 1-MeAde biosynthesis in follicle cells.",
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