Hormonal regulation of follicle-stimulating hormone receptors in the testes of Japanese quail

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    Abstract

    The effects of gonadotrophins and testosterone on the binding of 125I-labelled rat FSH to a testicular homogenate of hypophysectomized Japanese quail have been studied. Hypophysectomy resulted in rapid decrease in the binding of FSH per testis but did not change the binding per unit testicular weight, suggesting a decrease in FSH binding per Sertoli cell. Injections of NIH-FSH-S12 to hypophysectomized birds (100 μg/day) for 5 days induced hypertrophy of Sertoli cells and 2.2-fold increase in the binding of FSH per Sertoli cell. Injections of testosterone to hypophysectomized birds (5.0 mg/day) for 5 days also increased the binding of FSH per Sertoli cell 2-3-fold, but did not change the cell size. Combined administration of FSH and testosterone induced 4.4-fold increase in the binding of FSH per Sertoli cells and also marked hypertrophy. The Scatchard plot analysis of the binding of FSH to the testicular preparation of hypophysectomized birds treated simultaneously with FSH and testosterone showed that the number of the binding sites, but not the affinity of the binding, was increased by the hormone treatment. Synergistic action of FSH and testosterone was observed in the increase of not only the number of the FSH binding sites but also in testicular weight and the number of spermatogonia and spermatocytes. These results indicated that FSH receptors in the testes of Japanese quail are regulated by FSH and testosterone. It is suggested the FSH acting synergistically with testosterone increases the sensitivity of the testis to FSH itself by increasing FSH receptors. This self-potentiation mechanism may enable rapid testicular development in photo-stimulated birds and mammals at puberty.

    Original languageEnglish
    Pages (from-to)511-518
    Number of pages8
    JournalJournal of Endocrinology
    Volume85
    Issue number3
    Publication statusPublished - 1980

    Fingerprint

    FSH Receptors
    Coturnix
    Testosterone
    Testis
    Sertoli Cells
    Birds
    Hypertrophy
    Binding Sites
    Weights and Measures
    Hypophysectomy
    Spermatogonia
    Injections
    Spermatocytes
    Puberty
    Gonadotropins
    Cell Size
    Mammals
    Hormones

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Hormonal regulation of follicle-stimulating hormone receptors in the testes of Japanese quail. / Tsutsui, Kazuyoshi; Ishii, S.

    In: Journal of Endocrinology, Vol. 85, No. 3, 1980, p. 511-518.

    Research output: Contribution to journalArticle

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    abstract = "The effects of gonadotrophins and testosterone on the binding of 125I-labelled rat FSH to a testicular homogenate of hypophysectomized Japanese quail have been studied. Hypophysectomy resulted in rapid decrease in the binding of FSH per testis but did not change the binding per unit testicular weight, suggesting a decrease in FSH binding per Sertoli cell. Injections of NIH-FSH-S12 to hypophysectomized birds (100 μg/day) for 5 days induced hypertrophy of Sertoli cells and 2.2-fold increase in the binding of FSH per Sertoli cell. Injections of testosterone to hypophysectomized birds (5.0 mg/day) for 5 days also increased the binding of FSH per Sertoli cell 2-3-fold, but did not change the cell size. Combined administration of FSH and testosterone induced 4.4-fold increase in the binding of FSH per Sertoli cells and also marked hypertrophy. The Scatchard plot analysis of the binding of FSH to the testicular preparation of hypophysectomized birds treated simultaneously with FSH and testosterone showed that the number of the binding sites, but not the affinity of the binding, was increased by the hormone treatment. Synergistic action of FSH and testosterone was observed in the increase of not only the number of the FSH binding sites but also in testicular weight and the number of spermatogonia and spermatocytes. These results indicated that FSH receptors in the testes of Japanese quail are regulated by FSH and testosterone. It is suggested the FSH acting synergistically with testosterone increases the sensitivity of the testis to FSH itself by increasing FSH receptors. This self-potentiation mechanism may enable rapid testicular development in photo-stimulated birds and mammals at puberty.",
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