Housing under abnormal light-dark cycles attenuates day/night expression rhythms of the clock genes Per1, Per2, and Bmal1 in the amygdala and hippocampus of mice

Shunpei Moriya, Yu Tahara, Hiroyuki Sasaki, Jun Ishigooka, Shigenobu Shibata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although the results of previous studies have suggested that disruptions in circadian rhythms are involved in the pathogenesis of depression, no studies have examined the interaction of clock gene expression deficit and depression state. In this study, we examined clock gene expression levels and depressive-like behavior in mice housed under 3.5 h light, 3.5 h dark (T=7) conditions to investigate the association between clock gene expression and depressive state. C57BL/6J mice were housed under a T=24 cycle (12 h light, 12 h dark) or a T=7 cycle and clock gene expression levels in the hippocampus and the amygdala were measured by real-time RT-PCR. Depressive state was evaluated by the forced swim test (FST). Although circadian rhythms of Per1 and Per2 clock gene expression in the hippocampus and amygdala were still detected under T=7 conditions, rhythmicity and expression levels of both significantly decreased. Mice housed with a T=7 cycle showed increased immobile time in the FST than those with a T=24 cycle. The present results suggest that the presence of a depressive state around the early active phase of activity may be related to impairment of rhythmicity and expression levels of Per1 and Per2 genes under abnormal light-dark conditions.

Original languageEnglish
Pages (from-to)16-21
Number of pages6
JournalNeuroscience Research
Volume99
DOIs
Publication statusPublished - 2015 Oct 1

Fingerprint

Photoperiod
Amygdala
Hippocampus
Gene Expression
Genes
Periodicity
Circadian Rhythm
Light
Inbred C57BL Mouse
Real-Time Polymerase Chain Reaction

Keywords

  • Abnormal light cycle
  • Amygdala
  • Circadian rhythm
  • Clock gene
  • Depressive state

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Housing under abnormal light-dark cycles attenuates day/night expression rhythms of the clock genes Per1, Per2, and Bmal1 in the amygdala and hippocampus of mice. / Moriya, Shunpei; Tahara, Yu; Sasaki, Hiroyuki; Ishigooka, Jun; Shibata, Shigenobu.

In: Neuroscience Research, Vol. 99, 01.10.2015, p. 16-21.

Research output: Contribution to journalArticle

@article{e94e12f2d95140cfab606247bf343ab5,
title = "Housing under abnormal light-dark cycles attenuates day/night expression rhythms of the clock genes Per1, Per2, and Bmal1 in the amygdala and hippocampus of mice",
abstract = "Although the results of previous studies have suggested that disruptions in circadian rhythms are involved in the pathogenesis of depression, no studies have examined the interaction of clock gene expression deficit and depression state. In this study, we examined clock gene expression levels and depressive-like behavior in mice housed under 3.5 h light, 3.5 h dark (T=7) conditions to investigate the association between clock gene expression and depressive state. C57BL/6J mice were housed under a T=24 cycle (12 h light, 12 h dark) or a T=7 cycle and clock gene expression levels in the hippocampus and the amygdala were measured by real-time RT-PCR. Depressive state was evaluated by the forced swim test (FST). Although circadian rhythms of Per1 and Per2 clock gene expression in the hippocampus and amygdala were still detected under T=7 conditions, rhythmicity and expression levels of both significantly decreased. Mice housed with a T=7 cycle showed increased immobile time in the FST than those with a T=24 cycle. The present results suggest that the presence of a depressive state around the early active phase of activity may be related to impairment of rhythmicity and expression levels of Per1 and Per2 genes under abnormal light-dark conditions.",
keywords = "Abnormal light cycle, Amygdala, Circadian rhythm, Clock gene, Depressive state",
author = "Shunpei Moriya and Yu Tahara and Hiroyuki Sasaki and Jun Ishigooka and Shigenobu Shibata",
year = "2015",
month = "10",
day = "1",
doi = "10.1016/j.neures.2015.05.005",
language = "English",
volume = "99",
pages = "16--21",
journal = "Neuroscience Research",
issn = "0168-0102",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Housing under abnormal light-dark cycles attenuates day/night expression rhythms of the clock genes Per1, Per2, and Bmal1 in the amygdala and hippocampus of mice

AU - Moriya, Shunpei

AU - Tahara, Yu

AU - Sasaki, Hiroyuki

AU - Ishigooka, Jun

AU - Shibata, Shigenobu

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Although the results of previous studies have suggested that disruptions in circadian rhythms are involved in the pathogenesis of depression, no studies have examined the interaction of clock gene expression deficit and depression state. In this study, we examined clock gene expression levels and depressive-like behavior in mice housed under 3.5 h light, 3.5 h dark (T=7) conditions to investigate the association between clock gene expression and depressive state. C57BL/6J mice were housed under a T=24 cycle (12 h light, 12 h dark) or a T=7 cycle and clock gene expression levels in the hippocampus and the amygdala were measured by real-time RT-PCR. Depressive state was evaluated by the forced swim test (FST). Although circadian rhythms of Per1 and Per2 clock gene expression in the hippocampus and amygdala were still detected under T=7 conditions, rhythmicity and expression levels of both significantly decreased. Mice housed with a T=7 cycle showed increased immobile time in the FST than those with a T=24 cycle. The present results suggest that the presence of a depressive state around the early active phase of activity may be related to impairment of rhythmicity and expression levels of Per1 and Per2 genes under abnormal light-dark conditions.

AB - Although the results of previous studies have suggested that disruptions in circadian rhythms are involved in the pathogenesis of depression, no studies have examined the interaction of clock gene expression deficit and depression state. In this study, we examined clock gene expression levels and depressive-like behavior in mice housed under 3.5 h light, 3.5 h dark (T=7) conditions to investigate the association between clock gene expression and depressive state. C57BL/6J mice were housed under a T=24 cycle (12 h light, 12 h dark) or a T=7 cycle and clock gene expression levels in the hippocampus and the amygdala were measured by real-time RT-PCR. Depressive state was evaluated by the forced swim test (FST). Although circadian rhythms of Per1 and Per2 clock gene expression in the hippocampus and amygdala were still detected under T=7 conditions, rhythmicity and expression levels of both significantly decreased. Mice housed with a T=7 cycle showed increased immobile time in the FST than those with a T=24 cycle. The present results suggest that the presence of a depressive state around the early active phase of activity may be related to impairment of rhythmicity and expression levels of Per1 and Per2 genes under abnormal light-dark conditions.

KW - Abnormal light cycle

KW - Amygdala

KW - Circadian rhythm

KW - Clock gene

KW - Depressive state

UR - http://www.scopus.com/inward/record.url?scp=84940599465&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940599465&partnerID=8YFLogxK

U2 - 10.1016/j.neures.2015.05.005

DO - 10.1016/j.neures.2015.05.005

M3 - Article

C2 - 26026603

AN - SCOPUS:84940599465

VL - 99

SP - 16

EP - 21

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

ER -