How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets

Ryo Takagi, Shinichiro Kobayashi, Masayuki Yamato, Toshiyuki Owaki, Yoshiyuki Kasai, Takahiro Hosoi, Yusuke Sakai, Kengo Kanetaka, Tokutaro Minamizato, Asuka Minematsu, Makoto Kondo, Nobuo Kanai, Naoyuki Yamaguchi, Kazuhiro Nagai, Yasushi Miyazaki, Naoya Takeda, Fumio Fukai, Izumi Asahina, Taiga Miyazaki, Shigeru KohnoMasakazu Yamamoto, Kazuhiko Nakao, Susumu Eguchi, Teruo Okano

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    2 Citations (Scopus)

    Abstract

    We have utilized patients' own oral mucosa as a cell source for the fabrication of transplantable epithelial cell sheets to treat limbal stem cell deficiency and mucosal defects after endoscopic submucosal dissection of esophageal cancer. Because there are abundant microbiotas in the human oral cavity, the oral mucosa was sterilized and 40μg/mL gentamicin and 0.27μg/mL amphotericin B were added to the culture medium in our protocol. Although an oral surgeon carefully checked each patient's oral cavity and although candidiasis was not observed before taking the biopsy, contamination with Candida albicans (C.albicans) was detected in the conditioned medium during cell sheet fabrication. After adding 1μg/mL amphotericin B to the transportation medium during transport from Nagasaki University Hospital to Tokyo Women's Medical University, which are 1200km apart, no proliferation of C.albicans was observed. These results indicated that the supplementation of transportation medium with antimycotics would be useful for preventing contamination with C.albicans derived from the oral mucosa without hampering cell proliferation.

    Original languageEnglish
    Pages (from-to)1-4
    Number of pages4
    JournalRegenerative Therapy
    Volume1
    DOIs
    Publication statusPublished - 2015 Jun 1

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    Keywords

    • Amphotericin B
    • C.albicans
    • Candida albicans
    • DMEM
    • Oral mucosal epithelial cell

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Developmental Biology
    • Biomaterials

    Cite this

    Takagi, R., Kobayashi, S., Yamato, M., Owaki, T., Kasai, Y., Hosoi, T., Sakai, Y., Kanetaka, K., Minamizato, T., Minematsu, A., Kondo, M., Kanai, N., Yamaguchi, N., Nagai, K., Miyazaki, Y., Takeda, N., Fukai, F., Asahina, I., Miyazaki, T., ... Okano, T. (2015). How to prevent contamination with Candida albicans during the fabrication of transplantable oral mucosal epithelial cell sheets. Regenerative Therapy, 1, 1-4. https://doi.org/10.1016/j.reth.2014.12.002