How well can an amoeba climb?

Yoshio Fukui*, Taro Q.P. Uyeda, Chikako Kitayama, Shinya Inoué

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


We report here our efforts to measure the crawling force generated by cells undergoing amoeboid locomotion. In a centrifuge microscope, acceleration was increased until amoebae of Dictyostelium discoideum were "stalled" or no longer able to "climb up." The "apparent weight" of the amoebae at stalling rpm in myosin mutants depended on the presence of myosin II (but not myosins IA and IB) and paralleled the cortical strength of the cells. Surprisingly, however, the cell stalled not only In low-density media as expected but also in media with densities greater than the cell density where the buoyant force should push the amoeba upward. We find that the leading pseudopod Is bent under centrifugal force in all stalled amoebae, suggesting that this pseudopod is very dense Indeed. This finding also suggests that directional cell locomotion against resistive forces requires a turgid forward-pointing pseudopod, most likely sustained by cortical actomyosin II.

Original languageEnglish
Title of host publicationCollected Works of Shinya Inoue
Subtitle of host publicationMicroscopes, Living Cells, and Dynamic Molecules
PublisherWorld Scientific Publishing Co.
Number of pages6
ISBN (Electronic)9789812790866
ISBN (Print)9812703888, 9789812703880
Publication statusPublished - 2008 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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